Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt.
Division of International Infectious Diseases Control, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan.
Int J Nanomedicine. 2023 Mar 21;18:1335-1345. doi: 10.2147/IJN.S401876. eCollection 2023.
The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases.
The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis using surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics.
PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and 83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no statistical difference between PCR and nanogold ELISA results.
Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the assistance of nanoparticles, more antibodies enter the antigen-antibody complex because of the considerable improvement in the surface area of nano-gold particles.
Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies.
在孕妇中，准确诊断弓形虫病具有重要意义。纳米技术和分子生物学为许多传染病的准确和快速诊断提供了可能的机会。
我们的研究目的是比较纳米金 ELISA 与 ELISA 和 PCR 用于诊断门诊就诊的孕妇的弓形虫病表面抗原 1 级（SAG1）。
PCR 在敏感性（97.3%对 89.2%和 83.8%）、特异性（100%对 94%和 88%）和诊断准确性（98.9%对 91.95%和 86.2%）方面均高于纳米金 ELISA 和 ELISA。PCR 和纳米金 ELISA 结果之间无统计学差异。
纳米金 ELISA 比传统 ELISA 方法在诊断方面有显著提高。可能是由于纳米金颗粒的表面积大大提高，更多的抗体进入抗原抗体复合物。
虽然 PCR 的诊断价值高于纳米 ELISA，但纳米 ELISA 比 PCR 更便宜、更简单。我们建议使用 SAG1 的纳米金 ELISA 作为一种有前途的弓形虫病诊断技术和调查研究。
