Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, MN 55455, USA.
J Appl Microbiol. 2012 Dec;113(6):1540-53. doi: 10.1111/j.1365-2672.2012.05439.x. Epub 2012 Sep 17.
Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm model.
Saliva and dental plaque were collected from adults and children. Hydroxyapatite and dental composite discs were inoculated with either saliva or plaque, and microcosm biofilms were grown in a CDC biofilm reactor. In later experiments, the reactor was pulsed with sucrose. DNA from inoculums and microcosms was analysed by HOMIM for 272 species. Microcosms included about 60% of species from the original inoculum. Biofilms grown on hydroxyapatite and composites were extremely similar. Sucrose pulsing decreased diversity and pH, but increased the abundance of Streptococcus and Veillonella. Biofilms from the same donor, grown at different times, clustered together.
This model produced reproducible microcosm biofilms that were representative of the oral microbiota. Sucrose induced changes associated with dental caries.
This is the first use of HOMIM to validate an oral microcosm model that can be used to study the effects of complex biofilms on dental materials.
大多数关于生物膜对牙科材料影响的研究使用的是单一种群生物膜或联合体。直接从唾液或牙菌斑中生长的微宇宙生物膜更加多样化，但难以进行特征描述。我们使用人类口腔微生物鉴定微阵列（HOMIM）来验证可重现的口腔微宇宙模型。
从成年人和儿童中采集唾液和牙菌斑。将羟基磷灰石和牙科复合盘接种唾液或牙菌斑，并在 CDC 生物膜反应器中培养微宇宙生物膜。在后续实验中，反应器会被蔗糖脉冲刺激。通过 HOMIM 对接种物和微宇宙的 DNA 进行 272 种物种的分析。微宇宙包含原始接种物中约 60%的物种。羟基磷灰石和复合材料上生长的生物膜极为相似。蔗糖脉冲会降低多样性和 pH 值，但会增加链球菌和韦荣氏球菌的丰度。来自同一供体、在不同时间生长的生物膜聚集在一起。
该模型产生了可重现的微宇宙生物膜，可代表口腔微生物群。蔗糖诱导的变化与龋齿有关。
这是首次使用 HOMIM 验证可用于研究复杂生物膜对牙科材料影响的口腔微宇宙模型。
