Koji Uehara Emerson, Castro de Lima Gustavo, Sardi Janaina de Cassia Orlandi, de Figueiredo Luciene Cristina, Shibli Jamil Awad, Asbi Thabet, Haim Doron, Augusto Rodrigues José
Dental Research Division, Guarulhos University, Guarulhos 07023-070, SP, Brazil.
Maccabi-Dent Research Department, Tel-Aviv 6801298, Israel.
J Funct Biomater. 2025 Jan 3;16(1):12. doi: 10.3390/jfb16010012.
This study evaluated the microbial growth profile of subgingival multispecies biofilm on 3D-printable resin-based composites (PRBCs). A 96-well cell plate cultivated a 39-species biofilm associated with periodontitis over 7 days. Cylindrical specimens with 12 mm high and 3 mm diameters were prepared by the PRBC group (Cosmos Temp-Yller; Prizma 3D Bio Crown; Prizma 3D Bio Prov) and an acrylic resin as control. Further, these specimens were immersed in the well plate to allow biofilm formation. After growing for 7 days, the metabolic biofilm activity was evaluated by colorimetric assay and the microbial profile by DNA-DNA hybridization. Kruskal-Wallis and Mann-Whitney tests evaluated each bacteria count and complex group. A greater biofilm formation was observed on PRBC groups than on acrylic resin. The microbiological profile of PRBC was associated with a less pathogenic biofilm, with an absence of a red complex. Acrylic resin showed low biofilm growth, but the biofilm profile was related to periodontal disease, characterized by red-complex bacteria. The selection of PRBC may contribute more effectively to maintaining periodontal health than acrylic resin.
本研究评估了基于3D可打印树脂的复合材料（PRBC）上龈下多物种生物膜的微生物生长情况。一个96孔细胞培养板在7天内培养了与牙周炎相关的39种生物膜。PRBC组（Cosmos Temp-Yller；Prizma 3D生物冠；Prizma 3D生物桩）和作为对照的丙烯酸树脂制备了高12毫米、直径3毫米的圆柱形标本。此外，将这些标本浸入孔板中以形成生物膜。生长7天后，通过比色法评估代谢生物膜活性，并通过DNA-DNA杂交评估微生物情况。Kruskal-Wallis和Mann-Whitney检验评估了每种细菌计数和复合组。与丙烯酸树脂相比，PRBC组观察到更大的生物膜形成。PRBC的微生物情况与致病性较低的生物膜相关，不存在红色复合体。丙烯酸树脂显示出低生物膜生长，但生物膜情况与牙周疾病相关，其特征为红色复合体细菌。与丙烯酸树脂相比，选择PRBC可能更有效地有助于维持牙周健康。
