Gilbert P, Pemberton D, Wilkinson D E
Department of Pharmacy, University of Manchester, UK.
J Appl Bacteriol. 1990 Oct;69(4):593-8. doi: 10.1111/j.1365-2672.1990.tb01553.x.
Polyhexamethylene biguanides (PHMB) are mixtures of polymeric biguanides with an average polymer length (n) of 5, but containing high (n greater than 15, mol. wt 3300) and low molecular weight material (n = 2, mol. wt 400). Studies involving discrete molecular weight fractions of PHMB have shown that antimicrobial activity of PHMB increases with increasing polymer length. Cell suspensions which had not been subjected to centrifugation and/or washing during their preparation were employed. Whilst activity was still observed to increase with n, the trend was much reduced as n exceeded six. Centrifugation and washing of cells markedly increased the activity of high but not low molecular weight materials and corresponded to losses upon centrifugation of envelope lipopolysaccharide (LPS). Such envelope LPS represented high affinity binding sites on the surfaces of the cells. Combinations of various molecular weight fractions of PHMB were evaluated against filter-washed cells and revealed a profound synergy between extremes of polymer length.
聚六亚甲基双胍（PHMB）是平均聚合物长度（n）为5的聚合双胍混合物，但含有高分子量（n大于15，分子量3300）和低分子量物质（n = 2，分子量400）。涉及PHMB离散分子量级分的研究表明，PHMB的抗菌活性随聚合物长度增加而增强。制备过程中未进行离心和/或洗涤的细胞悬液被采用。虽然仍观察到活性随n增加，但当n超过6时，这种趋势大大减弱。细胞的离心和洗涤显著增加了高分子量而非低分子量物质的活性，并且与包膜脂多糖（LPS）离心后的损失相对应。这种包膜LPS代表细胞表面的高亲和力结合位点。评估了PHMB各种分子量级分对经过滤洗涤的细胞的组合，结果显示聚合物长度极端情况下存在显著的协同作用。
