凋亡信号调节激酶 1 与 claudin-6 在乳腺癌中的作用相关。
Apoptosis signal-regulating kinase 1 is associated with the effect of claudin-6 in breast cancer.
机构信息
The Key Laboratory of Pathobiology, Ministry of Education, Beyuthune Medical College, Jilin University, Changchun, Jilin, China.
出版信息
Diagn Pathol. 2012 Aug 27;7:111. doi: 10.1186/1746-1596-7-111.
BACKGROUND
Previous studies have demonstrated that claudin-6 functions as a cancer suppressor in human MCF-7 breast cancer cells. The growth inhibitory effect could be attributed to inhibition of cell proliferation and induction of apoptosis. The purpose of the current study was to examine the involvement of apoptosis signal-regulating kinase 1 (ASK1) in the anticancer effect of claudin-6.
METHODS
Immunohistochemical analysis was performed to evaluate the ASK1 protein expression and the correlation between ASK1, claudin-6 and clinicopathological features in 85 samples of breast invasive ductal carcinomas (IDC). Western blotting and RT-PCR was carried out to examine the expression of ASK1 and claudin-6 in MCF-7 cell clones transfected with claudin-6.
RESULTS
Immunohistochemical analysis showed that ASK1 expression was significantly related with that of claudin-6 in breast invasive ductal carcinomas (P < 0.05). In addition, a positive correlation between ASK1 and C-erb B 2 protein expression was identified (P < 0.05). Western blotting and RT-PCR consistently revealed that the level of ASK1 protein and mRNA was upregulated in MCF-7 cell clones transfected with claudin-6.
CONCLUSIONS
Our data suggests, for the first time, that the ASK1 signal may play a positive role in the inhibitory effect of claudin-6 in breast cancer.
VIRTUAL SLIDES
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1200314318763661.
背景
先前的研究表明，claudin-6 在人 MCF-7 乳腺癌细胞中作为一种肿瘤抑制因子发挥作用。这种生长抑制作用可以归因于细胞增殖的抑制和细胞凋亡的诱导。本研究的目的是研究凋亡信号调节激酶 1（ASK1）是否参与 claudin-6 的抗癌作用。
方法
采用免疫组织化学分析评估 85 例乳腺浸润性导管癌（IDC）中 ASK1 蛋白表达及其与 claudin-6 和临床病理特征之间的相关性。Western blot 和 RT-PCR 用于检测转染 claudin-6 的 MCF-7 细胞克隆中 ASK1 和 claudin-6 的表达。
结果
免疫组织化学分析显示，ASK1 表达与乳腺浸润性导管癌中的 claudin-6 表达显著相关（P<0.05）。此外，还发现 ASK1 与 C-erb B2 蛋白表达之间存在正相关（P<0.05）。Western blot 和 RT-PCR 一致显示，转染 claudin-6 的 MCF-7 细胞克隆中 ASK1 蛋白和 mRNA 的水平上调。
结论
我们的数据首次表明，ASK1 信号可能在 claudin-6 对乳腺癌的抑制作用中发挥积极作用。
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