MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Methods. 2013 Jan;59(1):101-7. doi: 10.1016/j.ymeth.2012.08.001. Epub 2012 Aug 19.
The efficient delivery of personalized medicine is a key goal of healthcare over the next decade. It is likely that PCR strategies will play an important role in the delivery of this goal. Digital PCR has certain advantages over more traditional PCR protocols. In this article we will discuss the current status of digital PCR, highlighting its advantages and focusing on how it can be utilized in biomarker development and analysis, including the use of individualized biomarkers. We will explore recent developments in this field including examples of how digital PCR may integrate with next generation sequencing to deliver truly personalized medicine.
在未来十年，个性化医疗的有效实施是医疗保健的主要目标。聚合酶链反应（PCR）策略很可能在实现这一目标中发挥重要作用。数字 PCR 相对于更传统的 PCR 方案具有某些优势。本文将讨论数字 PCR 的现状，突出其优势，并重点介绍其如何应用于生物标志物的开发和分析，包括个体化生物标志物的应用。我们将探讨该领域的最新进展，包括数字 PCR 如何与下一代测序相结合以提供真正的个性化医疗的实例。
