Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah 84112, USA.
Anal Chem. 2012 Oct 2;84(19):8323-9. doi: 10.1021/ac301424b. Epub 2012 Sep 12.
Characterization of polymerized liposomes (PolyPIPosomes) was carried out using a combination of normal dc electrical field-flow fractionation and cyclical electrical field-flow fractionation (CyElFFF) as an analytical technique. The constant nature of the carrier fluid and channel configuration for this technique eliminates many variables associated with multidimensional analysis. CyElFFF uses an oscillating field to induce separation and is performed in the same channel as standard dc electrical field-flow fractionation separation. Theory and experimental methods to characterize nanoparticles in terms of their sizes and electrophoretic mobilities are discussed in this paper. Polystyrene nanoparticles are used for system calibration and characterization of the separation performance, whereas polymerized liposomes are used to demonstrate the applicability of the system to biomedical samples. This paper is also the first to report separation and a higher effective field when CyElFFF is operated at very low applied voltages. The technique is shown to have the ability to quantify both particle size and electrophoretic mobility distributions for colloidal polystyrene nanoparticles and PolyPIPosomes.
采用常规直流电场流分离与循环电场流分离（CyElFFF）相结合的方法对聚合脂质体（PolyPIPosomes）进行了表征，该分析技术作为一种组合方法。该技术的载流液和通道结构保持恒定，消除了与多维分析相关的许多变量。CyElFFF 采用振荡场来诱导分离，并且与标准直流电场流分离在相同的通道中进行。本文讨论了用于表征纳米颗粒的尺寸和电泳迁移率的理论和实验方法。聚苯乙烯纳米颗粒用于系统校准和分离性能的表征，而聚合脂质体则用于证明该系统在生物医学样品中的适用性。本文也是首次报道在非常低的外加电压下操作 CyElFFF 时会出现分离和更高的有效场。该技术显示出对胶体聚苯乙烯纳米颗粒和 PolyPIPosomes 的粒径和电泳迁移率分布进行定量的能力。
