Kantak Ameya, Srinivas Merugu, Gale Bruce
University of Utah, Department of Mechanical Engineering, 50 S. Central Campus Drive Room 2110, Salt Lake City, UT 84112-9202, USA.
Lab Chip. 2006 May;6(5):645-54. doi: 10.1039/b516827a. Epub 2006 Mar 14.
A microscale cyclical electrical field flow fractionation (CyElFFF) channel is characterized with regard to the effect of various operating parameters and comparison made to recent theoretical developments. Challenges associated with various operating conditions are reported along with some of the optimized operating parameters. The effect of retention wall choice, an offset voltage, relaxation steps, and flow rates, along with the basic operating parameters of voltage, frequency, and electrophoretic mobility are reported. Retention of polystyrene nanoparticle standards is accomplished and the first separations using this technique in a microscale system are also demonstrated. Relaxation steps and offset voltages are found to be effective in eliminating early peaks and in improving plate heights. Plate heights were also found to decrease with increasing flow rates, which is the opposite of the behavior seen in most existing chromatographic systems. The experimental results are compared to the analytical and empirical models of CyElFFF and found to be compatible. Suggestions are made for improving the separation and analysis methods used with CyElFFF.
对微尺度循环电场流分级（CyElFFF）通道进行了表征，考察了各种操作参数的影响，并与近期的理论进展进行了比较。报告了与各种操作条件相关的挑战以及一些优化的操作参数。报道了保留壁选择、偏置电压、弛豫步骤和流速的影响，以及电压、频率和电泳迁移率等基本操作参数的影响。实现了聚苯乙烯纳米颗粒标准品的保留，并展示了在微尺度系统中首次使用该技术进行的分离。发现弛豫步骤和偏置电压在消除早期峰和改善塔板高度方面是有效的。还发现塔板高度随流速增加而降低，这与大多数现有色谱系统中的行为相反。将实验结果与CyElFFF的分析模型和经验模型进行了比较，发现两者是兼容的。针对改进CyElFFF所使用的分离和分析方法提出了建议。
