Department of Mechanical Engineering, University of Utah, Salt Lake City, UT 84112-9202, USA.
Electrophoresis. 2010 Oct;31(20):3372-9. doi: 10.1002/elps.201000024.
Cyclical electrical field flow fractionation (CyElFFF) is a variation on electrical field flow fractionation (ElFFF) where cyclical electrical fields are used instead of steady DC fields to increase the effective field experienced by particles in the flow channel. Even though the effective field increases more than 20-fold compared to normal ElFFF, the retention and resolution in CyElFFF has not been shown to be better than in ElFFF. In this paper we report how one can optimize operational parameters in CyElFFF to obtain good retention and resolution in CyElFFF. The effects of offset voltage, frequency, flowrate, concentration of particles and sample size on retention, resolution and retained peak/void peak ratio have been observed. The results obtained from these experiments were analyzed and suggestions have been made to improve both retention and resolution. A 4-fold improvement in retention without a significant increase in band broadening is reported.
循环电场流分离（CyElFFF）是电场流分离（ElFFF）的一种变体，其中使用循环电场代替稳定的直流场，以增加流道中颗粒所经历的有效场。尽管与普通 ElFFF 相比，有效场增加了 20 多倍，但 CyElFFF 的保留和分辨率并未显示优于 ElFFF。在本文中，我们报告了如何优化 CyElFFF 的操作参数以在 CyElFFF 中获得良好的保留和分辨率。观察了偏置电压、频率、流速、颗粒浓度和样品尺寸对保留、分辨率和保留峰/空隙峰比的影响。对这些实验结果进行了分析，并提出了改进保留和分辨率的建议。报道了在没有明显增加带宽展宽的情况下保留率提高了 4 倍。
