量子点在分析蛋白质组学中的应用赋予了其新颖性。

Quantum dot applications endowing novelty to analytical proteomics.

机构信息

Department of Chemistry, National Sun Yat Sen University, Kaohsiung, Taiwan.

出版信息

Proteomics. 2012 Oct;12(19-20):2949-61. doi: 10.1002/pmic.201200295.

DOI:10.1002/pmic.201200295

Abstract

This review surveys all the state-of-art applications of quantum dots (QDs) in conventional and modern analytical methods in proteomic studies. A brief introduction of QDs and their properties is initially presented followed by outlining the application of QDs in fluorescence, MS, imaging, and cancer-based proteomics. The in-depth application of QDs in MALDI-MS and surface assisted laser desorption/ionization-MS has been elaborately discussed, summarizing the speculated mechanism behind the protein-QDs interactions during QD matrix applications leading to enhanced detection sensitivity.

摘要

本综述调查了量子点（QD）在蛋白质组学研究中常规和现代分析方法中的所有最新应用。首先简要介绍了 QD 及其性质，然后概述了 QD 在荧光、MS、成像和基于癌症的蛋白质组学中的应用。详细讨论了 QD 在 MALDI-MS 和表面辅助激光解吸/电离-MS 中的深入应用，总结了在 QD 基质应用过程中蛋白质-QD 相互作用背后的推测机制，从而提高了检测灵敏度。