Hreash F, Laycock J F
Department of Physiology, Charing Cross and Westminster Medical School, UK.
Pflugers Arch. 1990 Sep;417(1):106-10. doi: 10.1007/BF00370777.
An extracorporeal circulation technique was developed for use in rats to provide equilibrated blood samples for multiple hormone assays. The inclusion of the extracorporeal circulation did not significantly alter arterial blood pressure, cardiac output, heart rate or central venous pressure in either Brattleboro rats with hereditary diabetes insipidus (BDI) or normal rats of the parent Long Evans (LE) strain. Plasma adrenaline and noradrenaline levels did not alter in either BDI or LE rats following inclusion of the extracorporeal circulation but the vasopressin concentration rose significantly in the LE rats. The impaired recovery of the mean arterial blood pressure following haemorrhage in the BDI rats compared with normal LE animals was not further influenced by the inclusion of the extracorporeal circulation. Plasma vasopressin and adrenaline (but not nor-adrenaline) levels were significantly raised during, and after, haemorrhage in the LE rats while in the BDI rats only plasma adrenaline levels were significantly increased. These results show that the insertion of an extracorporeal circulation into an anaesthetized BDI or LE rat does not adversely affect the cardiovascular system despite the increase in baseline plasma vasopressin concentration in normal rats, and its subsequent removal provides an additional equilibrated blood sample for multiple hormone assay within the same animal. The increased release of both adrenaline and vasopressin (but not noradrenaline) after haemorrhage in the same animal is detected using this technique, and the importance of vasopressin to the normal recovery process confirmed.
已开发出一种用于大鼠的体外循环技术，以提供用于多种激素测定的平衡血样。在患有遗传性尿崩症的布拉德福德大鼠（BDI）或亲代长埃文斯（LE）品系的正常大鼠中，加入体外循环并未显著改变动脉血压、心输出量、心率或中心静脉压。加入体外循环后，BDI大鼠和LE大鼠的血浆肾上腺素和去甲肾上腺素水平均未改变，但LE大鼠的血管加压素浓度显著升高。与正常LE动物相比，BDI大鼠出血后平均动脉血压恢复受损的情况并未因加入体外循环而进一步受到影响。LE大鼠在出血期间和出血后血浆血管加压素和肾上腺素（而非去甲肾上腺素）水平显著升高，而在BDI大鼠中，仅血浆肾上腺素水平显著增加。这些结果表明，尽管正常大鼠的基线血浆血管加压素浓度升高，但将体外循环插入麻醉的BDI或LE大鼠不会对心血管系统产生不利影响，并且随后移除体外循环可为同一动物提供用于多种激素测定的额外平衡血样。使用该技术可检测到同一动物出血后肾上腺素和血管加压素（而非去甲肾上腺素）释放增加，并且证实了血管加压素对正常恢复过程的重要性。
