Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Department of Clinical Laboratory and Medical Research Center, Zhuhai Hospital of Jinan University, Zhuhai, China.
J Clin Lab Anal. 2019 Nov;33(9):e22981. doi: 10.1002/jcla.22981. Epub 2019 Jul 19.
Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross-reaction or true sensitization.
Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross-reactivity was analyzed and determined by sIgE inhibition with 21 sera samples.
Around 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co-sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe-level (class 5-6) co-sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups C < C and C < C .
This study has established valuable information about the co-sensitization and cross-reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite-allergic patients.
布氏嗜鳞螨（Blo t）、屋尘螨（Der p）和粉尘螨（Der f）之间的交叉反应分析结果此前存在矛盾。本研究旨在探讨它们之间共致敏的特征和 IgE 交叉反应，并试图确定患者是否因交叉反应而致敏于 Blo t，还是真正致敏。
通过 ImmunoCAP 测定 1497 例变应原患者血清中的特异性 IgE（sIgE）。通过 21 例血清样本的 sIgE 抑制分析来确定交叉反应。
约 85.50%的患者对 Der p 致敏，85.37%的患者对 Der f 致敏，71.54%的患者对 Blo t 致敏。此外，70.14%的患者对 Blo t、Der p 和 Der f 共致敏，仅有 7 例患者仅对 Blo t 致敏。随着 Blo t 的 sIgE 水平增加，对 Der p 或 Der f 的重度（5-6 级）共致敏的阳性率显著增加。Blo t 与 Der p 和 Der f 中度相关，相关系数分别为 0.6998 和 0.6782。在 C < C 和 C < C 组中，Der p 和 Der f 抑制 IgE 与 Blo t 的结合比 Blo t 抑制 IgE 与 Der p 或 Der f 的结合更强。
本研究为布氏嗜鳞螨与两种屋尘螨（Der p 和 Der f）之间的共致敏和交叉反应提供了有价值的信息，有助于为螨过敏患者提供充分的诊断和治疗。
