• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

产碳青霉烯酶肠杆菌科的快速检测。

Rapid detection of carbapenemase-producing Enterobacteriaceae.

机构信息

Service de Bactériologie-Virologie, Hôpital de Bicêtre 78 Rue du Général Leclerc, 94275 K-Bicêtre, France.

出版信息

Emerg Infect Dis. 2012 Sep;18(9):1503-7. doi: 10.3201/eid1809.120355.

DOI:10.3201/eid1809.120355
PMID:22932472
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3437707/
Abstract

To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.

摘要

为了快速鉴定肠杆菌科中的碳青霉烯酶产生菌,我们开发了 Carba NP 检测方法。该检测方法使用分离的细菌菌落,基于碳青霉烯类药物亚胺培南的体外水解。与基于分子的技术相比,它具有 100%的灵敏度和特异性。这种快速(<2 小时)、廉价的技术可在任何实验室实施。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d01/3437707/6ba1fa683ae9/12-0355-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d01/3437707/ab7a764e4b3c/12-0355-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d01/3437707/6ba1fa683ae9/12-0355-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d01/3437707/ab7a764e4b3c/12-0355-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d01/3437707/6ba1fa683ae9/12-0355-F2.jpg

相似文献

1
Rapid detection of carbapenemase-producing Enterobacteriaceae.产碳青霉烯酶肠杆菌科的快速检测。
Emerg Infect Dis. 2012 Sep;18(9):1503-7. doi: 10.3201/eid1809.120355.
2
Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae.自制碳青霉烯酶检测(Carba-NP)试验与蓝碳青霉烯酶检测(Blue-Carba)试验的比较:提高产碳青霉烯酶肠杆菌科细菌检测效果的考量
J Microbiol Methods. 2016 Mar;122:33-7. doi: 10.1016/j.mimet.2016.01.002. Epub 2016 Jan 7.
3
Detection of Carbapenemase Production in a Collection of Enterobacteriaceae with Characterized Resistance Mechanisms from Clinical and Environmental Origins by Use of Both Carba NP and Blue-Carba Tests.通过使用Carba NP试验和Blue-Carba试验检测来自临床和环境来源、具有明确耐药机制的肠杆菌科细菌中碳青霉烯酶的产生情况。
J Clin Microbiol. 2016 Feb;54(2):464-6. doi: 10.1128/JCM.02580-15. Epub 2015 Nov 25.
4
Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae.评估用于生化检测产碳青霉烯酶肠杆菌科细菌的RAPIDEC® CARBA NP、Rapid CARB Screen®和Carba NP检测方法。
J Antimicrob Chemother. 2015 Nov;70(11):3014-22. doi: 10.1093/jac/dkv213. Epub 2015 Aug 9.
5
Comparative Study of a Novel Biochemical Assay, the Rapidec Carba NP Test, for Detecting Carbapenemase-Producing Enterobacteriaceae.用于检测产碳青霉烯酶肠杆菌科细菌的新型生化检测方法——Rapidec Carba NP试验的比较研究
J Clin Microbiol. 2016 Feb;54(2):453-6. doi: 10.1128/JCM.02626-15. Epub 2015 Nov 18.
6
Rapid detection of carbapenemase-producing Enterobacteriaceae from blood cultures.血培养中碳青霉烯酶产生肠杆菌科的快速检测。
Clin Microbiol Infect. 2014 Apr;20(4):340-4. doi: 10.1111/1469-0691.12318. Epub 2013 Jul 24.
7
Evaluation of Carba NP test for rapid detection of carbapenemase producing Enterobacteriaceae.用于快速检测产碳青霉烯酶肠杆菌科细菌的Carba NP试验评估
Indian J Med Microbiol. 2015 Oct-Dec;33(4):603-6. doi: 10.4103/0255-0857.167332.
8
Phenotypic and molecular detection methods for carbapenemase-producing organisms and their clinical significance at two Scottish tertiary care hospitals.产碳青霉烯酶菌的表型和分子检测方法及其在苏格兰两家三级保健医院的临床意义。
J Med Microbiol. 2019 Apr;68(4):560-565. doi: 10.1099/jmm.0.000931. Epub 2019 Mar 14.
9
Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test.使用改良版Carba NP试验检测产碳青霉烯酶肠杆菌科细菌时,分离培养基的影响
J Med Microbiol. 2014 May;63(Pt 5):772-776. doi: 10.1099/jmm.0.071340-0. Epub 2014 Mar 3.
10
The Problem of Carbapenemase-Producing-Carbapenem-Resistant-Enterobacteriaceae Detection.产碳青霉烯酶的耐碳青霉烯肠杆菌科细菌检测问题
J Clin Microbiol. 2016 Mar;54(3):529-34. doi: 10.1128/JCM.02771-15. Epub 2016 Jan 6.

引用本文的文献

1
Cefiderocol-resistant pathogens in German hospital wastewater: a reservoir for multidrug resistance.德国医院废水中对头孢地尔耐药的病原体:多重耐药性的一个储存库。
Sci Rep. 2025 Aug 27;15(1):31622. doi: 10.1038/s41598-025-17379-2.
2
The genetic context of blaIMP varies among bacterial families from One Health sources.blaIMP的基因背景在来自“同一健康”来源的不同细菌家族中有所不同。
PLoS One. 2025 Jul 23;20(7):e0327200. doi: 10.1371/journal.pone.0327200. eCollection 2025.
3
The impact of zinc supplementation on carbapenem MICs among bacteria expressing IMP metallo-beta-lactamase.

本文引用的文献

1
Overview of Changes to the Clinical and Laboratory Standards Institute M100, 31st Edition.临床和实验室标准协会 M100,31 版更改概述。
J Clin Microbiol. 2021 Nov 18;59(12):e0021321. doi: 10.1128/JCM.00213-21. Epub 2021 Sep 22.
2
Identification and screening of carbapenemase-producing Enterobacteriaceae.碳青霉烯酶肠杆菌科的鉴定和筛选。
Clin Microbiol Infect. 2012 May;18(5):432-8. doi: 10.1111/j.1469-0691.2012.03815.x.
3
Are susceptibility tests enough, or should laboratories still seek ESBLs and carbapenemases directly?
补充锌对表达IMP金属β-内酰胺酶的细菌中碳青霉烯类最低抑菌浓度的影响。
Access Microbiol. 2025 Jun 26;7(6). doi: 10.1099/acmi.0.000972.v4. eCollection 2025.
4
Development and evaluation of 1,4,7-triazacyclononane-coupled β-lactams against metallo-β-lactamase producing bacteria.针对产金属β-内酰胺酶细菌的1,4,7-三氮杂环壬烷偶联β-内酰胺的研发与评估
RSC Adv. 2025 Jul 7;15(29):23427-23440. doi: 10.1039/d5ra01842k. eCollection 2025 Jul 4.
5
Antimicrobial resistance and beta-lactamase gene distribution among clinical isolates: a two-year cohort study.临床分离株中的抗菌药物耐药性和β-内酰胺酶基因分布:一项为期两年的队列研究。
Sci Rep. 2025 Jul 4;15(1):23951. doi: 10.1038/s41598-025-08445-w.
6
Challenges of Carbapenem-Resistant in the Development of New β-Lactamase Inhibitors and Antibiotics.耐碳青霉烯类在新型β-内酰胺酶抑制剂和抗生素研发中的挑战
Antibiotics (Basel). 2025 Jun 7;14(6):587. doi: 10.3390/antibiotics14060587.
7
Combined resistance mechanisms leading to high-level of cefiderocol resistance among NDM-like producing E. coli ST167 clinical isolates.导致产NDM样的大肠杆菌ST167临床分离株对头孢地尔产生高水平耐药的联合耐药机制。
Eur J Clin Microbiol Infect Dis. 2025 May 27. doi: 10.1007/s10096-025-05166-w.
8
Multidrug-resistant gram-negative bacteria in ICU patients of a tertiary care hospital in Saudi Arabia: distribution of carbapenemase genes in clinical and rectal swab samples.沙特阿拉伯一家三级护理医院重症监护病房患者中的多重耐药革兰氏阴性菌:临床和直肠拭子样本中碳青霉烯酶基因的分布
Naunyn Schmiedebergs Arch Pharmacol. 2025 Apr 24. doi: 10.1007/s00210-025-04194-9.
9
Saccharin disrupts bacterial cell envelope stability and interferes with DNA replication dynamics.糖精会破坏细菌细胞壁的稳定性,并干扰DNA复制动态。
EMBO Mol Med. 2025 May;17(5):993-1017. doi: 10.1038/s44321-025-00219-1. Epub 2025 Apr 1.
10
Small-Molecule Probes: Recent Progress in the Rapid Detection of Carbapenemase-Producing Bacteria.小分子探针:产碳青霉烯酶细菌快速检测的最新进展
Pharmaceutics. 2025 Feb 20;17(3):282. doi: 10.3390/pharmaceutics17030282.
药敏试验就足够了吗,还是实验室仍应直接检测超广谱β-内酰胺酶(ESBLs)和碳青霉烯酶?
J Antimicrob Chemother. 2012 Jul;67(7):1569-77. doi: 10.1093/jac/dks088. Epub 2012 Mar 29.
4
Global spread of Carbapenemase-producing Enterobacteriaceae.产碳青霉烯酶肠杆菌科的全球传播。
Emerg Infect Dis. 2011 Oct;17(10):1791-8. doi: 10.3201/eid1710.110655.
5
The emergence of pan-resistant Gram-negative pathogens merits a rapid global political response.泛耐药革兰氏阴性病原体的出现需要全球迅速做出政治反应。
J Antimicrob Chemother. 2012 Jan;67(1):1-3. doi: 10.1093/jac/dkr378. Epub 2011 Oct 12.
6
Combating antimicrobial resistance: policy recommendations to save lives.抗击抗菌素耐药性:拯救生命的政策建议。
Clin Infect Dis. 2011 May;52 Suppl 5(Suppl 5):S397-428. doi: 10.1093/cid/cir153.
7
NDM-1--a cause for worldwide concern.新德里金属-β-内酰胺酶-1——一个引起全球关注的因素。
N Engl J Med. 2010 Dec 16;363(25):2377-9. doi: 10.1056/NEJMp1011715.
8
How to detect NDM-1 producers.如何检测 NDM-1 产生菌。
J Clin Microbiol. 2011 Feb;49(2):718-21. doi: 10.1128/JCM.01773-10. Epub 2010 Dec 1.
9
Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase - United States, 2010.检出携带金属β-内酰胺酶的肠杆菌科分离株-美国,2010 年。
MMWR Morb Mortal Wkly Rep. 2010 Jun 25;59(24):750.
10
Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid.通过加入硼酸来控制霍奇和増田检测肠杆菌科细菌 A 类碳青霉烯酶时产生的假阳性结果。
J Clin Microbiol. 2010 Apr;48(4):1323-32. doi: 10.1128/JCM.01771-09. Epub 2010 Feb 24.