Lode Anja, Meissner Katrin, Luo Yongxiang, Sonntag Frank, Glorius Stefan, Nies Berthold, Vater Corina, Despang Florian, Hanke Thomas, Gelinsky Michael
Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Medical Faculty of Technische Universität Dresden, Germany; Max Bergmann Centre of Biomaterials, Technische Universität Dresden, Institute for Materials Science, Dresden, Germany.
J Tissue Eng Regen Med. 2014 Sep;8(9):682-93. doi: 10.1002/term.1563. Epub 2012 Aug 30.
The major advantage of hydroxyapatite (HA)-forming calcium phosphate cements (CPCs) used as bone replacement materials is their setting under physiological conditions without the necessity for thermal treatment that allows the incorporation of biological factors. In the present study, we have combined the biocompatible consolidation of CPCs with the potential of rapid prototyping (RP) techniques to generate calcium phosphate-based scaffolds with defined inner and outer morphology. We demonstrate the application of the RP technique three-dimensional (3D) plotting for the fabrication of HA cement scaffolds. This was realized by utilizing a paste-like CPC (P-CPC) which is stable as a malleable paste and whose setting reaction is initiated only after contact with aqueous solutions. The P-CPC showed good processability in the 3D plotting process and allowed the fabrication of stable 3D structures of different geometries with adequate mechanical stability and compressive strength. The cytocompatibility of the plotted P-CPC scaffolds was demonstrated in a cell culture experiment with human mesenchymal stem cells. The mild conditions during 3D plotting and post-processing and the realization of the whole procedure under sterile conditions make this approach highly attractive for fabrication of individualized implants with respect to patient-specific requirements by simultaneous plotting of biological components.
用作骨替代材料的形成羟基磷灰石（HA）的磷酸钙骨水泥（CPC）的主要优点是它们在生理条件下凝固，无需热处理，这使得生物因子得以掺入。在本研究中，我们将CPC的生物相容性固化与快速成型（RP）技术的潜力相结合，以生成具有确定内部和外部形态的磷酸钙基支架。我们展示了RP技术三维（3D）绘图在HA水泥支架制造中的应用。这是通过使用一种糊状CPC（P-CPC）实现的，它作为一种可延展的糊状物是稳定的，并且其固化反应仅在与水溶液接触后才开始。P-CPC在3D绘图过程中显示出良好的加工性能，并允许制造具有足够机械稳定性和抗压强度的不同几何形状的稳定3D结构。在用人骨髓间充质干细胞进行的细胞培养实验中证明了绘制的P-CPC支架的细胞相容性。3D绘图和后处理过程中的温和条件以及在无菌条件下实现整个过程，使得这种方法对于通过同时绘制生物成分来制造符合患者特定要求的个性化植入物极具吸引力。
