Liu Ru, Zhao Yangguo, Lu Shanshan, Huang Qing
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Wei Sheng Wu Xue Bao. 2012 Jun 4;52(6):744-52.
Two-chamber microbial fuel cells (MFCs) were set up to understand the electrogenic capacity of MFCs fed with lactic acid to investigate the distribution characteristics of microflora in the anode biofilm, supernatant, and sediment.
Using lactic acid as a carbon source in the anode, we explored the MFCs start-up process and the efficiency of electricity production, and also investigated the spatial distribution of microbial communities using scanning electron microscope (SEM) and PCR-denaturing gradient gel electrophoresis (DGGE) techniques.
The results indicate that the MFCs reached the highest voltage, 0.56 V on the seventh day after startup. When external resistance and current density was 80 omega and 415 mA/m2, respectively, the power density reached its maximum at 82 mW/m2. SEM revealed that a massive bacillus was attached tightly to the surface of the positive electrode. DGGE profiles revealed that microorganisms on the anode's surface were most similar to that of inoculated sludge, consistent with the major microorganism groups in anode suspension and sludge substrate. Communities developed on the anodes included exoelectrogenic bacteria, i. e. Comamonas testosterone, and Arcobacter butzleri.
This research demonstrates that MFCs fed with lactic acid can generate a high efficiency of current density, and that the dominant microbes on the anodes are similar to that of inoculated sludge.
搭建双室微生物燃料电池（MFCs），以了解以乳酸为燃料的MFCs的产电能力，研究阳极生物膜、上清液和沉积物中微生物群落的分布特征。
在阳极中使用乳酸作为碳源，探索MFCs的启动过程和产电效率，并使用扫描电子显微镜（SEM）和聚合酶链反应-变性梯度凝胶电泳（DGGE）技术研究微生物群落的空间分布。
结果表明，MFCs在启动后第7天达到最高电压0.56V。当外部电阻和电流密度分别为80Ω和415mA/m²时，功率密度在82mW/m²时达到最大值。SEM显示大量杆菌紧密附着在正极表面。DGGE图谱显示阳极表面的微生物与接种污泥的微生物最为相似，这与阳极悬浮液和污泥底物中的主要微生物群一致。阳极上形成的群落包括产电细菌，即睾酮丛毛单胞菌和布氏弓形杆菌。
本研究表明，以乳酸为燃料的MFCs能够产生高效的电流密度，且阳极上的优势微生物与接种污泥的优势微生物相似。
