在突尼斯的马和蜱虫中发现嗜吞噬细胞无形体。
Anaplasma phagocytophilum in horses and ticks in Tunisia.
机构信息
Laboratoire d'Épidémiologie et Microbiologie Vétérinaire, Service d'Entomologie Médicale, Institut Pasteur de Tunis, Tunis 1002, Tunisia.
出版信息
Parasit Vectors. 2012 Aug 30;5:180. doi: 10.1186/1756-3305-5-180.
BACKGROUND
Anaplasma phagocytophilum , the causative agent of granulocytic anaplasmosis, affects several species of wild and domesticated mammals, including horses. We used direct and indirect methods to compare and evaluate exposure to A. phagocytophilum in horses in northern Tunisia.
METHODS
Serum from 60 horses was tested by IFA for antibodies to A. phagocytophilum , and whole blood was tested for A. phagocytophilum 16S rRNA gene using a nested-PCR. To examine the risk of A. phagocytophilum transmission, 154 ticks that had been collected from horses were examined for the presence of A. phagocytophilum by nested-PCR targeting 16S rRNA gene.
RESULTS
This is the first time that A. phagocytophilum has been detected in horses in Tunisia, with an overall seroprevalence of 40/60 (67%). Six of the seroreactive samples (10%) had an IFA titer of 1:80, 14 (23%) of 1:160, 8 (13%) of 1:320 and 12 (20%) a titer 1 ≥ 640. The seroprevalence revealed no significant regional and sex differences. In contrast, a significant difference was observed between breeds. Eight (13%) of the horses were positive for A. phagocytophilum in the PCR, with no significant breed and age differences. Hyalomma marginatum was a predominant tick species (130/154), and 3 were infected by A. phagocytophilum (a prevalence of 2.3%). The concordance rate of A. phagocytophilum detection between IFA and PCR had a k value of -0.07.
CONCLUSIONS
The results presented in this study suggest that horses infested by ticks in Tunisia are exposed to A. phagocytophilum.
背景
嗜吞噬细胞无形体是粒细胞无形体病的病原体，可感染包括马在内的多种野生动物和家养动物。我们采用直接和间接方法比较和评估了突尼斯北部马匹感染嗜吞噬细胞无形体的情况。
方法
使用间接免疫荧光法（IFA）检测 60 匹马的血清抗体，巢式 PCR 检测全血中的嗜吞噬细胞无形体 16S rRNA 基因。为了研究嗜吞噬细胞无形体传播的风险，使用巢式 PCR 检测了从马身上采集的 154 只蜱虫中是否存在嗜吞噬细胞无形体 16S rRNA 基因。
结果
这是首次在突尼斯的马匹中检测到嗜吞噬细胞无形体，总血清阳性率为 40/60（67%）。6 份血清学阳性样本（10%）的 IFA 滴度为 1:80，14 份（23%）为 1:160，8 份（13%）为 1:320，12 份（20%）为 1 ≥ 640。血清学阳性率在地域和性别上无显著差异，但在品种间存在显著差异。PCR 检测有 8 匹马（13%）呈嗜吞噬细胞无形体阳性，在品种和年龄上无显著差异。边缘革蜱是主要的蜱种（130/154），其中 3 只感染了嗜吞噬细胞无形体（感染率为 2.3%）。IFA 和 PCR 检测嗜吞噬细胞无形体的一致性率 k 值为-0.07。
结论
本研究结果表明，突尼斯受蜱虫感染的马匹可能接触过嗜吞噬细胞无形体。
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