Salvagni Carlos Augusto, Dagnone Ana Sílvia, Gomes Tiago Salles, Mota Jozivaldo Silva, Andrade Gisele Maria, Baldani Cristiane Divan, Machado Rosangela Zacarias
Departamento de Patologia Veterinária, FCAV/UNESP, Universidade Estadual Paulista-UNESP.
Rev Bras Parasitol Vet. 2010 Jul-Sep;19(3):135-40. doi: 10.1590/s1984-29612010000300002.
Ehrlichiosis is a zoonotic disease caused by gram-negative and intracellular obligatory bacterial organisms. Equine Granulocytic Anaplasmosis - EGA (formerly Equine Granulocytic Ehrlichiosis, EGE) is a seasonal disease, normally self-limited in horses. There are few reports in Brazil about this ehrlichial agent, as well as its natural vectors. Nowadays, veterinarians are considering the suspicion of EGA in horses with suggestive symptoms of ehrlichiosis and which do not respond to piroplasmosis treatment. The aim of the present study was to identify horses exposed to the agent A. phagocytophilum by serological and molecular techniques. Twenty equine blood and serum samples from the central West region of Brazil were evaluated by microscopic examination of buffy coat smear, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR). Additionally, the serodiagnosis of Theileria equi by IFA and ELISA were carried out, as well as molecular diagnosis by nPCR. Thirteen (65%) serum samples were positive for A. phagocytophilum by ELISA, but none of them were positive by buffy-coat smear examination or nPCR. Antibodies IgG anti-T. equi were detected in 18 (90%) and 17 (85%) horses by IFA and ELISA, respectively and the agent was detected in 9 (45%) animals by nPCR. Our data may be considered as important information to understanding the occurrence of EGA and equine piroplasmosis in central West Brazil.
埃立克体病是一种由革兰氏阴性细胞内专性细菌病原体引起的人畜共患病。马粒细胞无形体病（EGA，以前称为马粒细胞埃立克体病，EGE）是一种季节性疾病，通常在马中为自限性。在巴西，关于这种埃立克体病原体及其天然传播媒介的报道很少。如今，兽医们在怀疑患有埃立克体病提示症状且对梨形虫病治疗无反应的马匹中考虑EGA。本研究的目的是通过血清学和分子技术鉴定接触嗜吞噬细胞无形体病原体的马匹。对来自巴西中西部地区的20份马血和血清样本进行了血沉棕黄层涂片显微镜检查、酶联免疫吸附测定（ELISA）、间接荧光抗体试验（IFAT）和巢式聚合酶链反应（nPCR）评估。此外，还通过IFA和ELISA对马泰勒虫进行了血清学诊断，并通过nPCR进行了分子诊断。通过ELISA检测，13份（65%）血清样本对嗜吞噬细胞无形体呈阳性，但血沉棕黄层涂片检查或nPCR均未发现阳性样本。通过IFA和ELISA分别在18匹（90%）和17匹（85%）马中检测到抗马泰勒虫IgG抗体，通过nPCR在9只（45%）动物中检测到该病原体。我们的数据可被视为了解巴西中西部地区EGA和马梨形虫病发生情况的重要信息。
