Plant Genetics Institute, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.
Am J Bot. 2012 Sep;99(9):e362-5. doi: 10.3732/ajb.1200064. Epub 2012 Aug 30.
Pinus pinea is one of the few widespread plant species that are also genetically depauperate. It is also an important commercial species with high market value seeds. A deeper knowledge of the existing population genetic variation was needed.
Twelve nuclear microsatellites were isolated from genomic and cDNA sequences and screened for variability in 729 individuals from 33 natural populations. Low level of genetic variability was confirmed with average expected heterozygosity of 0.11. Hardy-Weinberg equilibrium expectations were not met in only ∼10% of the possible locus/population combinations. All loci were in linkage equilibrium, and the frequency of null alleles was very low (≤1% in 332 out of 396 locus/population combinations). Nine out of the 12 microsatellites were successfully transferred to P. halepensis.
Despite low polymorphism, these new markers will be useful to resolve population structure and hold potential for seed origin identification and traceability.
地中海松是少数几种分布广泛但遗传资源匮乏的植物物种之一。它也是一种具有高市场价值种子的重要商业物种。因此，需要更深入地了解现有的种群遗传变异。
从基因组和 cDNA 序列中分离出 12 个核微卫星，并对来自 33 个自然种群的 729 个个体进行了变异性筛选。平均预期杂合度为 0.11，证实了遗传变异性水平较低。仅在约 10%的可能的位点/种群组合中不符合 Hardy-Weinberg 平衡预期。所有位点均处于连锁平衡状态，且无效等位基因的频率非常低（在 396 个位点/种群组合中，有 332 个的无效等位基因频率≤1%）。12 个微卫星中有 9 个成功转移到欧洲赤松中。
尽管多态性较低，但这些新标记物将有助于解决种群结构问题，并具有用于种子起源鉴定和可追溯性的潜力。
