Baygi Modjtaba Emadi, Nikpour Parvaneh
Department of Genetics, Faculty of Basic Sciences, Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
Asian Pac J Cancer Prev. 2012;13(6):2833-6. doi: 10.7314/apjcp.2012.13.6.2833.
Gastric cancer is the third most frequent cause of cancer mortality worldwide. In Iran, it is one of the leading causes at the national level. Localized at chromosome 8q22, the human MTDH gene has been reported to be over-expressed in a spectrum of malignancies. However, since there is a lack of data concerning with expression in gastric cancer at the transcriptional level, in this study we evaluated MTDH expression in Iranian cases.
Totally, thirty paired gastric samples were examined by quantitative real-time RT-PCR.
Although the mRNA expression was significantly elevated in 46.6% of the examined tumor tissues; its expression was low in others (36.6%). Moreover, there was only a marginal statistical difference between the MTDH gene expression of all tumor specimens compared to their paired non-tumor ones and no statistically significant association with the grades and types of the tumors.
Taken together, our results demonstrated that expression of MTDH at the transcriptional level may be increased in gastric cancer tissue samples but with considerable heterogeneity. Due to this, it may have the potential to be used as a target for diagnostic/therapeutic purposes only in a subset of patients.
胃癌是全球癌症死亡的第三大常见原因。在伊朗，它是国家层面的主要病因之一。人类MTDH基因定位于染色体8q22，据报道在一系列恶性肿瘤中过度表达。然而，由于缺乏转录水平上胃癌表达的数据，在本研究中我们评估了伊朗病例中MTDH的表达。
总共通过定量实时逆转录聚合酶链反应检测了30对胃样本。
尽管在所检测的肿瘤组织中46.6%的样本mRNA表达显著升高；但其他样本（36.6%）表达较低。此外，与配对的非肿瘤样本相比，所有肿瘤标本的MTDH基因表达仅存在微小的统计学差异，且与肿瘤的分级和类型无统计学显著关联。
综上所述，我们的结果表明，MTDH在转录水平的表达在胃癌组织样本中可能升高，但具有相当大的异质性。因此，它可能仅在一部分患者中具有用作诊断/治疗靶点的潜力。
