Emadi-Andani Elaheh, Nikpour Parvaneh, Emadi-Baygi Modjtaba, Bidmeshkipour Ali
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
Department of Genetics and Molecular Biology, Faculty of Medicine, Pediatric Inherited Diseases Research Center, Child Growth and Development Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2014 May 28;3:135. doi: 10.4103/2277-9175.133278. eCollection 2014.
Gastric cancer is the second and fourth most common cancer in Iranian men and women, respectively, but it is the first leading cause of cancer deaths in Iran. Most Iranian patients with gastric cancer are diagnosed at an advanced stage of disease when the conventional treatments have no effect on improving the survival. So, early gastric cancer detection is of high priority in order to decrease its high mortality rate in Iran. HOTAIR is a long non-coding RNA which its overexpression has been documented in different types of human cancer and can be considered as a potential cancer biomarker. The aim of this study was to evaluate the clinicopathological relevance of the expression of HOTAIR gene in gastric carcinoma.
A total of 60 tumoral and non-tumoral gastric specimens were evaluated for HOTAIR gene expression using quantitative real-time PCR.
The expression of HOTAIR was markedly increased in gastric cancer tissues compared with adjacent non-tumoral tissues. We further showed that there was a positive significant correlation between the HOTAIR gene expression, TNM staging, perineural invasion, and distant metastasis, but not with other clinicopathological features of gastric tumors.
These results suggest that HOTAIR expression is modulated during gastric cancer progression and therefore may participate in molecular processes relevant to malignant transformation and metastasis in gastric carcinoma.
胃癌分别是伊朗男性和女性中第二和第四常见的癌症，但它是伊朗癌症死亡的首要原因。大多数伊朗胃癌患者在疾病晚期被诊断出来，此时传统治疗方法对提高生存率无效。因此，为降低伊朗胃癌的高死亡率，早期胃癌检测至关重要。HOTAIR是一种长链非编码RNA，其过表达已在不同类型的人类癌症中得到证实，可被视为一种潜在的癌症生物标志物。本研究的目的是评估HOTAIR基因表达在胃癌中的临床病理相关性。
使用定量实时PCR对60份肿瘤性和非肿瘤性胃标本进行HOTAIR基因表达评估。
与相邻的非肿瘤组织相比，胃癌组织中HOTAIR的表达明显增加。我们进一步表明，HOTAIR基因表达与TNM分期、神经周围浸润和远处转移之间存在显著正相关，但与胃肿瘤的其他临床病理特征无关。
这些结果表明，HOTAIR表达在胃癌进展过程中受到调节，因此可能参与了与胃癌恶性转化和转移相关的分子过程。
