Higham S M, Edgar W M
Department of Dental Sciences, School of Dental Surgery, Liverpool, UK.
Caries Res. 1990;24(1):39-43. doi: 10.1159/000261236.
The effect of supplementing the diet or drinking water with lactate dehydrogenase (LDH) on the formation of caries lesions in rats was investigated. Addition of LDH [20 and 5 mg/kg in the high-sucrose (65%) diet, 2 and 1 U/ml in the drinking water (LDH-specific activity 735 U/mg solid)] resulted in significant reductions (p less than 0.01) in the number and severity of caries lesions when the enzyme was administered to rats for 4 or 7 days, although no significant differences were found after 14 or 21 days. No differences were observed between effects of addition to water or diet. Fissure caries failed to develop when rats were fed a low sucrose diet (5%) containing LDH (20 mg/kg diet), whereas 50% of the animals in the control group developed lesions. The findings indicate that LDH may play an important role in delaying the onset of caries lesion formation in rats, and that the protection observed may be inversely related to the intensity of the cariogenic challenge.
研究了在大鼠饮食或饮用水中补充乳酸脱氢酶（LDH）对龋齿病变形成的影响。添加LDH[在高糖（65%）饮食中为20和5mg/kg，在饮用水中为2和1U/ml（LDH比活性735U/mg固体）]，当给大鼠施用该酶4或7天时，龋齿病变的数量和严重程度显著降低（p<0.01），尽管在14或21天后未发现显著差异。添加到水中或饮食中的效果之间未观察到差异。当给大鼠喂食含有LDH（20mg/kg饮食）的低糖饮食（5%）时，窝沟龋未发生，而对照组中50%的动物出现了病变。研究结果表明，LDH可能在延缓大鼠龋齿病变形成的起始过程中起重要作用，并且观察到的保护作用可能与致龋挑战的强度呈负相关。
