Department of Food Life Science, Konkuk University, Chungju 380-701, Republic of Korea.
Bioorg Med Chem Lett. 2012 Oct 1;22(19):6136-8. doi: 10.1016/j.bmcl.2012.08.030. Epub 2012 Aug 14.
In this study we propose a novel anticancer agent using hetero-chitooligosaccharide (hetero-COS). To examine the possibility of the hetero-COS as a anticancer agent, we prepared nine kinds of hetero-COS with relatively higher molecular weights (90, 75 and 50-COS I, 5-10kDa), medium molecular weights (90, 75 and 50-COS II, 1-5kDa), and lower molecular weights (90, 75 and 50-III, below 1kDa), and their anticancer properties were investigated on HL-60 cells using flow cytometry and morphological analysis. The results obtained indicate that 90-COS III, which is relatively higher degree of deacetylation and lower molecular weights, showed the highest anticancer activity, and the data showed the anticancer property of the hetero-COSs depended on their degree of deacetylation values and molecular weight.
在这项研究中，我们提出了一种使用杂合壳寡糖（hetero-COS）的新型抗癌剂。为了检验 hetero-COS 作为抗癌剂的可能性，我们制备了九种具有相对较高分子量（90、75 和 50-COS I，5-10kDa）、中等分子量（90、75 和 50-COS II，1-5kDa）和低分子量（90、75 和 50-III，低于 1kDa）的 hetero-COS，并使用流式细胞术和形态分析研究了它们在 HL-60 细胞上的抗癌特性。结果表明，具有相对较高脱乙酰度和较低分子量的 90-COS III 表现出最高的抗癌活性，数据表明 hetero-COS 的抗癌特性取决于其脱乙酰度值和分子量。
