Rodríguez-Hernandez J, Canut-Blasco A, Ledesma-Garcia M, Martín-Sánchez A M
Departamento de Microbiologia, Universidad de Salamanca, Spain.
Eur J Epidemiol. 1994 Apr;10(2):215-8. doi: 10.1007/BF01730373.
A study was carried out on the presence of oocysts of Cryptosporidium spp. in water for human consumption in the Province of Salamanca, Spain, using four different staining techniques: Ziehl-Neelsen, auramine, indirect immunofluorescence with monoclonal antibodies (Meridian, USA) and direct immunofluorescence with monoclonal antibodies (Pasteur, France). The presence of oocysts was detected in the water used for the washing of sand filters in the four water purifying plants and in the drinking water of two of the six geographical areas studied. The Ziehl-Neelsen and auramine staining techniques were found to have excellent diagnostic correlation. Immunofluorescence stainings with monoclonal antibodies proved to be less sensitive.
在西班牙萨拉曼卡省，使用四种不同的染色技术，即齐-尼氏染色法、金胺染色法、用单克隆抗体的间接免疫荧光法（美国子午线公司）和用单克隆抗体的直接免疫荧光法（法国巴斯德公司），对供人类饮用的水中隐孢子虫属卵囊的存在情况进行了一项研究。在所研究的六个地理区域中的四个水净化厂用于冲洗砂滤器的水中以及两个区域的饮用水中检测到了卵囊。发现齐-尼氏染色法和金胺染色法具有极好的诊断相关性。用单克隆抗体的免疫荧光染色法被证明敏感性较低。
