Miller K J, Gore R S, Johnson R, Benesi A J, Reinhold V N
Department of Food Science, Pennsylvania State University, University Park 16802.
J Bacteriol. 1990 Jan;172(1):136-42. doi: 10.1128/jb.172.1.136-142.1990.
We report the initial characterization of the cell-associated oligosaccharides produced by four Bradyrhizobium strains: Bradyrhizobium japonicum USDA 110, USDA 94, and ATCC 10324 and Bradyrhizobium sp. strain 32H1. The cell-associated oligosaccharides of these strains were found to be composed solely of glucose and were predominantly smaller than the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species. Linkage studies and nuclear magnetic resonance analyses demonstrated that the bradyrhizobial glucans are linked primarily by beta-1,6 and beta-1,3 glycosidic bonds. Thus, the bradyrhizobia appear to synthesize cell-associated oligosaccharides of structural character substantially different from that of the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species.
日本慢生根瘤菌USDA 110、USDA 94、ATCC 10324以及慢生根瘤菌属菌株32H1。发现这些菌株的细胞相关寡糖仅由葡萄糖组成，并且主要比土壤杆菌属和根瘤菌属产生的环状β-1,2-葡聚糖小。连接研究和核磁共振分析表明，慢生根瘤菌葡聚糖主要通过β-1,6和β-1,3糖苷键连接。因此，慢生根瘤菌似乎合成了结构特征与土壤杆菌属和根瘤菌属产生的环状β-1,2-葡聚糖有很大不同的细胞相关寡糖。
