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细胞传代培养对哺乳动物细胞紫外线增强病毒复活的影响。

An effect of cell-culture passage on ultraviolet-enhanced viral reactivation by mammalian cells.

作者信息

Moore S P, Coohill T P

出版信息

Mutat Res. 1979 Oct;62(3):417-23. doi: 10.1016/0027-5107(79)90037-x.

DOI:10.1016/0027-5107(79)90037-x
PMID:229411
Abstract

The ability of ultraviolet-irradiated African green monkey kidney cells to reactivate UV-damaged herpes simplex virus was tested over a range of 57 passages (24-81). Several types of controls were employed to minimize daily variations. The results indicate that in this system cells demonstrate an increasing efficiency to reactivate UV-damaged virus until approx. passage 60, followed by a decrease in this ability beyond this passage. Chromosome counts at selected passages revealed no significant change in chromosome number as a function of passage. The mechanism(s) or factor(s) causing this effect are unknown.

摘要

在57代(24 - 81代)的范围内,测试了紫外线照射的非洲绿猴肾细胞重新激活紫外线损伤的单纯疱疹病毒的能力。采用了几种类型的对照来尽量减少每日变化。结果表明,在这个系统中,细胞重新激活紫外线损伤病毒的效率不断提高,直到大约第60代,之后这一能力下降。在选定代次进行的染色体计数显示,染色体数量并未随代次发生显著变化。导致这种效应的机制或因素尚不清楚。

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