School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
Carbohydr Polym. 2012 Nov 6;90(4):1494-500. doi: 10.1016/j.carbpol.2012.07.020. Epub 2012 Jul 14.
Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration of 59 nmol/mg polymer to increase cell-biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3-5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall, CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing.
组织修复是创伤启动的自发过程。然而，如果这一复杂机制受损或不足，生物材料的使用可能会增加成功愈合的机会。有鉴于此，开发了一种 RGD 功能化聚合物以促进皮肤愈合。合成了一种水溶性壳聚糖衍生物羧甲基-三甲基壳聚糖（CM-TM-壳聚糖），并将 GRGDS 部分接枝到主链上，浓度为 59 nmol/mg 聚合物，以增加细胞-生物材料相互作用。在体外与培养的人真皮成纤维细胞进行测试，所开发的聚合物表现出良好的生物相容性，由于 GRGDS 部分的存在，初始粘附增加了 3-5 倍。此外，细胞铺展与 GRGDS 的相互作用有关，在 30 分钟内，有 12 倍的细胞呈现完全铺展形态。总体而言，与 GRGDS 肽偶联的 CM-TM-壳聚糖可用作伤口愈合的生物材料。
