Wongpanit Panya, Sanchavanakit Neeracha, Pavasant Prasit, Supaphol Pitt, Tokura Seiichi, Rujiravanit Ratana
Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand.
Macromol Biosci. 2005 Oct 20;5(10):1001-12. doi: 10.1002/mabi.200500081.
CM-chitin and CM-chitosan films were successfully crosslinked by microwave treatment. Crosslinking of the microwave-treated CM-chitin films involved mainly the carboxylate and the secondary alcohol groups, while crosslinking of microwave-treated CM-chitosan films involved the carboxylate and the amino groups. In addition, the crystallinity of CM-chitin increased with increasing microwave treatment time, whereas an increase in the crystallinity of the microwave-treated CM-chitosan films was not observed. At a similar percentage of weight loss, the crosslinking of either CM-chitin or CM-chitosan films by microwave treatment required much less stringent condition when compared with the crosslinking by autoclave treatment. Based on both direct and indirect cytotoxicity assays, the cytotoxicity of the microwave-treated CM-chitin films was negative, while that of the microwave-treated CM-chitosan films was positive. Human fibroblasts adhered on the surface of microwave-treated CM-chitosan films much better than on the surface of microwave-treated CM-chitin films.
通过微波处理成功地使羧甲基甲壳素（CM-甲壳素）和羧甲基壳聚糖（CM-壳聚糖）薄膜发生交联。微波处理的CM-甲壳素薄膜的交联主要涉及羧酸盐和仲醇基团，而微波处理的CM-壳聚糖薄膜的交联涉及羧酸盐和氨基。此外，CM-甲壳素的结晶度随微波处理时间的增加而增加，而未观察到微波处理的CM-壳聚糖薄膜的结晶度增加。在相似的失重百分比下，与高压釜处理交联相比，通过微波处理使CM-甲壳素或CM-壳聚糖薄膜交联所需的条件要宽松得多。基于直接和间接细胞毒性试验，微波处理的CM-甲壳素薄膜的细胞毒性为阴性，而微波处理的CM-壳聚糖薄膜的细胞毒性为阳性。人成纤维细胞在微波处理的CM-壳聚糖薄膜表面的粘附比在微波处理的CM-甲壳素薄膜表面要好得多。
