Anstead Michael, Heltshe Sonya L, Khan Umer, Barbieri Joseph T, Langkamp Markus, Döring Gerd, Dharia Shimoni, Gibson Ronald L, Treggiari Miriam M, Lymp James, Rosenfeld Margaret, Ramsey Bonnie
Department of Pediatrics, University of Kentucky, Lexington KY 40563-0284, USA.
Seattle Children's Hospital and University of Washington School of Medicine, Seattle WA 98105-0371, USA.
J Cyst Fibros. 2013 Mar;12(2):147-53. doi: 10.1016/j.jcf.2012.08.001. Epub 2012 Sep 1.
The prognostic value of Pseudomonas aeruginosa serology for antibiotic therapy in cystic fibrosis patients is not well understood.
Using five antigens from two ELISAs, we assessed whether positive serology in CF patients participating in the multi-center Early Pseudomonas Infection in Children (EPIC) trial would predict treatment failure, time to pulmonary exacerbation and risk for recurrent P. aeruginosa isolation post eradication.
Baseline positive P. aeruginosa serology was not significantly associated with failure of initial P. aeruginosa eradication measured at week 10 (adjusted for baseline culture) but seropositivity to the antigens alkaline protease and exotoxin A was significantly associated with increased risk for recurrent P. aeruginosa isolation during the 60 week post eradication follow-up period (p=0.003 and p=0.001 respectively). There was no association between baseline seropositivity and time to pulmonary exacerbation.
P. aeruginosa serology may complement culture results in clinicians' efforts to successfully monitor recurrence of early P. aeruginosa in CF patients.
铜绿假单胞菌血清学对囊性纤维化患者抗生素治疗的预后价值尚未完全明确。
我们使用来自两种酶联免疫吸附测定（ELISA）的五种抗原，评估参与多中心儿童早期铜绿假单胞菌感染（EPIC）试验的囊性纤维化患者血清学阳性是否能预测治疗失败、肺部恶化时间以及根除后铜绿假单胞菌再次分离的风险。
基线时铜绿假单胞菌血清学阳性与第10周时初始铜绿假单胞菌根除失败（根据基线培养情况进行调整）无显著相关性，但对碱性蛋白酶和外毒素A抗原的血清阳性与根除后60周随访期间铜绿假单胞菌再次分离风险增加显著相关（分别为p = 0.003和p = 0.001）。基线血清阳性与肺部恶化时间之间无关联。
铜绿假单胞菌血清学可能有助于临床医生成功监测囊性纤维化患者早期铜绿假单胞菌复发，补充培养结果。
