Environmental Proteomics Laboratory, Department of Biological Sciences, Center for Coastal Marine Science, California Polytechnic State University, San Luis Obispo, CA 93407-0401, USA.
Integr Comp Biol. 2012 Nov;52(5):622-5. doi: 10.1093/icb/ics116. Epub 2012 Sep 2.
The study of the proteome in response to environmental change is beginning to generate a number of new hypotheses about how organisms respond and adapt to a variety of stressors. The contributions to this symposium highlight how comparisons at the levels of species, populations, and tissues provide exciting new perspectives on the diversity of biochemical responses involved in the tolerance of stress. Despite limited genomic information, a number of studies of nonmodel organisms provide insights that are only accessible through a systems approach like proteomics. The realization that these systemic responses differ among closely related species, populations, and tissues illustrates the potential importance of the proteome to an organism's evolutionary response to a rapidly changing environment. Changes in an organism's proteome may occur as early as during the first stages of development and continue through acclimatization of the adult and adaptation of the following generations. A proteomic approach can also demonstrate how pollutants have systemic effects that may be counter-intuitive to expectations, emphasizing how isolating a single mode of action for a pollutant, e.g., xeno-androgen, is often inadequate. To continue with the progress made, we need a critical evaluation of the experimental designs used in proteomics studies, a reevaluation of some of the statistical analyses, and new technical advances in order to identify a greater number of proteins. The contributions to the current symposium offer the novice a starting point for assessing the potential of proteomics to generate novel hypotheses about how organisms interact with their environment.
该研究的蛋白质组对环境变化开始产生一些新的假说，关于如何生物应对和适应各种压力。专题讨论会的贡献强调如何比较在物种，种群和组织水平提供了令人兴奋的新观点的生物化学反应的多样性涉及压力的容忍。尽管基因组信息有限，一些非模式生物的研究提供了只有通过系统的方法，如蛋白质组学才能获得的见解。认识到这些系统反应在密切相关的物种、种群和组织之间存在差异，说明了蛋白质组对于生物体对快速变化的环境的进化反应的潜在重要性。生物体蛋白质组的变化可能早在发育的早期阶段就发生，并持续到成年的适应和后代的适应。蛋白质组学方法还可以证明污染物如何具有可能与预期相反的系统效应，强调将污染物的单一作用模式（例如，外源性雄激素）孤立起来通常是不够的。为了继续取得进展，我们需要对蛋白质组学研究中使用的实验设计进行批判性评估，重新评估一些统计分析，并在新技术方面取得进展，以便能够识别出更多的蛋白质。当前专题讨论会的贡献为新手提供了一个起点，用于评估蛋白质组学在产生关于生物体如何与其环境相互作用的新假设方面的潜力。
