Key Laboratory of Environmental Exposure and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China; Institute of Orthopedic Diseases, Department of Bone and Joint Surgery, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China.
Key Laboratory of Environmental Exposure and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China.
Sci Total Environ. 2021 Sep 20;788:147588. doi: 10.1016/j.scitotenv.2021.147588. Epub 2021 May 7.
The connection among genome expression, proteome alteration, metabolism regulation and phenotype change under environmental stresses is very vague. It is a tough task for the traditional research approaches to reveal the related scientific mechanisms of the above connection at molecular and systematic levels. Proteomics approach is an insightful tool for revealing the biological functions, metabolic networks and functional protein interaction networks of cells and organisms under stresses at the systematic level. The purpose of this review is to provide an insightful guideline on how to set up a proteomic investigation for revealing biomolecule mechanisms, protein biomarkers and metabolism networks related to stress response, pollutant recognition, transport and biodegradation, and providing an insightful high-throughput approach for screening functional enzymes and effective microbes based on bioinformatics and functional verification method. Furthermore, the toxicity evaluation of pollutants and byproducts by proteomics approaches provides a scientific insight for early diagnosis of ecological risk and determination of the effectiveness of pollutant treatment techniques.
在环境胁迫下，基因组表达、蛋白质组变化、代谢调控和表型变化之间的联系非常模糊。传统的研究方法很难揭示分子和系统水平上这种联系的相关科学机制。蛋白质组学方法是揭示细胞和生物体在应激条件下的生物学功能、代谢网络和功能蛋白相互作用网络的有效工具。本综述的目的是为如何开展蛋白质组学研究提供一个有见地的指导，以揭示与应激反应、污染物识别、运输和生物降解相关的生物分子机制、蛋白质生物标志物和代谢网络，并基于生物信息学和功能验证方法为筛选功能酶和有效微生物提供一种高通量的筛选方法。此外，蛋白质组学方法还可用于评估污染物及其副产物的毒性，为早期诊断生态风险和确定污染物处理技术的效果提供科学依据。
