Ye Hua, Huang Yan, Wang Jinjin, Peng Zuogang, Zhang Yaoguang
The Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Beibei, Chongqing 400715, PR China.
Mitochondrial DNA. 2013 Feb;24(1):34-6. doi: 10.3109/19401736.2012.716053. Epub 2012 Sep 5.
The golden Chinese loach, Sinibotia superciliaris (Günther, 1892) is an endemic fish in China, which is only distributed in the main river and tributaries of the upper and middle Yangtze River. In this study, we sequenced two mitochondrial genomes simultaneously from two samples of S. superciliaris collected from two different locations. Both mitogenomes were 16,572 bp in length, showing a typical vertebrate mitogenome feature with 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and 1 major non-coding control region (CR). Alignment and comparative analyses showed that the two mitogenome sequences possessed 22 mutation sites, all of which were transitions and mainly occurred in protein-coding genes (13/22) and CR (7/22). Among the 13 mutation sites that occurred in protein-coding genes, three mutation sites occurred in the first codon position and the remaining 10 sites occurred in the third codon position. However, only one mutation that occurred in the first codon position resulted in non-synonymous substitution in COI gene. The molecular data presented in this study will contribute to further phylogenetic analyses for this group of fishes.
中华金沙鳅（Sinibotia superciliaris (Günther, 1892)）是中国特有的鱼类，仅分布于长江中上游的干流及支流。在本研究中，我们同时对采自两个不同地点的中华金沙鳅样本进行了两个线粒体基因组的测序。两个线粒体基因组长度均为16,572 bp，呈现出典型的脊椎动物线粒体基因组特征，包含13个蛋白质编码基因、22个转运RNA、2个核糖体RNA和1个主要的非编码控制区（CR）。比对和比较分析表明，这两个线粒体基因组序列共有22个突变位点，均为转换，主要发生在蛋白质编码基因（13/22）和CR（7/22）中。在蛋白质编码基因中出现的13个突变位点中，3个突变位点发生在第一密码子位置，其余10个位点发生在第三密码子位置。然而，仅第一密码子位置发生的一个突变导致了COI基因中的非同义替换。本研究提供的分子数据将有助于对该类鱼类进行进一步的系统发育分析。
