Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China.
J Virol Methods. 2012 Dec;186(1-2):43-8. doi: 10.1016/j.jviromet.2012.08.007. Epub 2012 Aug 27.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
建立并优化了一种逆转录环介导等温扩增（RT-LAMP）检测方法，用于检测牛病毒性腹泻病毒（BVDV）RNA。该 RT-LAMP 检测法具有高度的灵敏度，能够检测到 4.67×10(0)拷贝的 BVDV RNA。此外，该 RT-LAMP 方法能够检测到两种基因型的 BVDV。与其他牛病毒无交叉反应。该 RT-LAMP 法还能够检测从牛粪便拭子中提取的 BVDV RNA。在 88 份粪便拭子中，38 份经 RT-LAMP 检测呈阳性，而 39 份经实时 RT-PCR 检测呈阳性。综上所述，BVDV 特异性 RT-LAMP 方法具有高度的特异性和敏感性，可用于检测临床样本的快速直接诊断方法。
