AD Center, Prague Psychiatric Center, Ustavni 91, 181 03 Praha 8, Bohnice, Czech Republic.
J Neuroimmunol. 2012 Nov 15;252(1-2):100-5. doi: 10.1016/j.jneuroim.2012.08.001. Epub 2012 Sep 2.
The role of humoral immunity related to neuron- and disease-specific cytoskeletal proteins patients with Alzheimer disease (AD) is unknown. We measured antibodies against tau protein, light and heavy (NFH) neurofilaments using ELISA in 80 paired serum and cerebrospinal fluid (CSF) samples from patients with AD, with other dementias (OD), with neuro-inflammatory diseases (IC) and 25 controls (NC). We estimated intrathecal synthesis according to the formula (CSF/serum anti-neurocytoskeletal IgG)/(CSF/serum total IgG). AD patients had significantly higher intrathecal anti-tau and anti-NFH antibodies than the other groups. These innovative findings may hint at specific alterations in humoral anti-neurocytoskeletal immunity and selectivity in AD, which could have diagnostic and immunotherapeutic implications.
阿尔茨海默病（AD）患者体液免疫与神经元和疾病特异性细胞骨架蛋白的关系尚不清楚。我们使用 ELISA 法检测了 80 对 AD 患者、其他痴呆（OD）患者、神经炎症性疾病（IC）患者和 25 名对照者的血清和脑脊液（CSF）中针对 tau 蛋白、轻和重（NFH）神经丝的抗体。我们根据公式（CSF/血清抗神经细胞骨架 IgG）/（CSF/血清总 IgG）来估计鞘内合成。AD 患者的鞘内抗 tau 和抗 NFH 抗体明显高于其他组。这些创新性发现可能提示 AD 患者体液抗神经细胞骨架免疫存在特异性改变和选择性，这可能具有诊断和免疫治疗意义。
