LightLab Imaging, Inc., Westford, MA 01886, USA.
IEEE Trans Biomed Eng. 2012 Dec;59(12):3386-95. doi: 10.1109/TBME.2012.2215858. Epub 2012 Aug 28.
We introduce a method to automatically extract spike features of the AIDS virus imaged through an electron microscope. The AIDS virus spike is the primary target of drug design as it is directly involved in infecting host cells. Our method detects the location of these spikes and extracts a subvolume enclosing the spike. We have achieved a sensitivity of 80% for our best operating range. The extracted spikes are further aligned and combined to build a 4-D statistical shape model, where each voxel in the shape model is assigned a probability density function. Our method is the first fully automated technique that can extract subvolumes of the AIDS virus spike and be used to build a statistical model without the need for any user supervision. We envision that this new tool will significantly enhance the overall process of shape analysis of the AIDS virus spike imaged through the electron microscope. Accurate models of the virus spike will help in the development of better drug design strategies.
我们介绍了一种自动提取电子显微镜下艾滋病病毒刺突特征的方法。艾滋病病毒的刺突是药物设计的主要靶点，因为它直接参与感染宿主细胞。我们的方法检测这些刺突的位置，并提取一个包含刺突的子体积。在我们的最佳工作范围内，我们的方法达到了 80%的灵敏度。提取的刺突进一步对齐和组合，以构建一个 4-D 统计形状模型，其中形状模型中的每个体素都被分配一个概率密度函数。我们的方法是第一个完全自动化的技术，可以提取艾滋病病毒刺突的子体积，并用于构建统计模型，而无需任何用户监督。我们设想这个新工具将极大地增强电子显微镜下艾滋病病毒刺突形状分析的整体过程。准确的病毒刺突模型将有助于开发更好的药物设计策略。
