寡聚态与蛋白质功能之间的关系。

The relationship between oligomeric state and protein function.

机构信息

Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Melbourne, Victoria, Australia.

出版信息

Adv Exp Med Biol. 2012;747:74-90. doi: 10.1007/978-1-4614-3229-6_5.

Abstract

The reason that many proteins adopt a particular oligomeric form is far from obvious. In this chapter, we discuss potential advantages of proteins self-assembling into specific quaternary structures. A number of case studies are presented in which wild-type proteins have been mutated to generate variants of lower oligomeric order and the impact on the resulting proteins, in terms of both specific function and generic stability, are discussed. Drawing on these case studies, some general design principles for quaternary structure engineering are put forward to facilitate these experiments on a wider range of systems. It is clear that the advantages afforded by quaternary structure vary from protein to protein; however, some general trends are starting to emerge.

摘要

许多蛋白质采用特定的寡聚形式的原因远非显而易见。在本章中，我们讨论了蛋白质自组装成特定四级结构的潜在优势。我们提出了一些案例研究，其中野生型蛋白质发生突变产生了低寡聚态的变体，并讨论了这些变体对特定功能和普遍稳定性的影响。借鉴这些案例研究，提出了一些用于四级结构工程的设计原则，以更广泛的系统中进行这些实验。显然，四级结构带来的优势因蛋白质而异；然而，一些普遍的趋势正在开始显现。