Kim Geon A, Oh Hyun Ju, Park Jung Eun, Kim Min Jung, Park Eun Jung, Lim Sang Hyun, Kang Sung Keun, Jang Goo, Lee Byeong Chun
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-744, Korea.
Reprod Fertil Dev. 2013;25(4):700-6. doi: 10.1071/RD11221.
It has been suggested that co-transferring parthenogenetic embryos could improve the pregnancy success rate with cloned embryos in mammals. As an alternative to co-transferring parthenotes, in dogs we employed recipient females that possessed in vivo-fertilised embryos as a result of mating to determine whether mated bitches could be suitable recipients for cloned embryos. The effect of using mated recipients on implantation and pregnancy rates of canine somatic cell nuclear transfer embryos was also determined. Cloned embryos were transferred into the oviducts of naturally synchronous females that had mated with male dogs before ovulation. The pregnancy rate appeared to be similar between mated recipients (50%) and non-mated recipients (28.57%; P>0.05). However, the delivery rate of cloned pups was significantly higher in mated recipients than non-mated recipients (10.53 vs 2.38%; P<0.05). A decrease in progesterone levels in the mated recipients before the due date induced natural delivery. However, cloned pups in non-mated recipients were delivered by Caesarean section because the fall in progesterone concentration in these females did not occur until the due date. The present study demonstrated for the first time that mated female dogs can be used as recipients for cloned embryos.
有人提出，共同移植孤雌生殖胚胎可以提高哺乳动物克隆胚胎的妊娠成功率。作为共同移植孤雌生殖胚胎的替代方法，在犬类中，我们使用了因交配而体内受精的雌性受体，以确定已交配的母犬是否适合作为克隆胚胎的受体。还确定了使用已交配受体对犬体细胞核移植胚胎着床率和妊娠率的影响。将克隆胚胎移植到在排卵前与公犬交配过的自然同步的雌性动物的输卵管中。已交配受体的妊娠率（50%）与未交配受体的妊娠率（28.57%；P>0.05）似乎相似。然而，已交配受体的克隆幼犬分娩率显著高于未交配受体（10.53%对2.38%；P<0.05）。预产期前已交配受体的孕酮水平下降导致自然分娩。然而，未交配受体中的克隆幼犬通过剖腹产分娩，因为这些雌性动物的孕酮浓度直到预产期才下降。本研究首次证明已交配的雌性犬可以用作克隆胚胎的受体。
