Department of Animal Sciences, University of Illinois, Urbana 61801, USA.
J Anim Sci. 2013 Jun;91(6):2802-11. doi: 10.2527/jas.2012-5327. Epub 2012 Sep 5.
Dried fermentation biomass (DFB) and hydrolyzed porcine intestinal mucosa are co-products of L-Lys • HCl production and heparin extraction, respectively. Three experiments were conducted to determine standardized ileal digestibility (SID) of AA (Exp. 1), concentration of DE and ME (Exp. 2), and standardized total tract digestibility (STTD) of P (Exp. 3) in DFB and 2 hydrolyzed porcine intestinal mucosa products (PEP50 and PEP2+), and compare these values with values for fish meal. In Exp. 1, 12 ileal cannulated barrows (BW = 11.5 ± 1.1 kg) were allotted to a replicated 6 × 6 Latin square design with 6 diets and 6 periods. A N-free diet, diet based on soybean meal (SBM), and 4 diets based on a combination of SBM and DFB, PEP50, PEP2+, or fish meal were formulated. With the exception of Lys, there were no differences in SID of indispensable AA between DFB and fish meal. Except for Thr, no differences in SID of indispensable AA between PEP50 and fish meal were observed, but SID of all indispensable AA, except Lys and Trp, was less (P < 0.05) in PEP2+ than in the other ingredients. In Exp. 2, 40 barrows (BW = 12.8 ± 1.4 kg) were allotted to 5 diets with 8 pigs/diet. A basal diet containing 96.4% corn and 4 diets containing corn and DFB, PEP50, PEP2+, or fish meal were formulated. The DE (5,445 kcal/kg DM) and ME (5,236 kcal/kg DM) in DFB were greater (P < 0.01) than in PEP50 (4,758 and 4,512 kcal/kg DM for DE and ME, respectively) and fish meal (4,227 and 3,960 kcal/kg DM for DE and ME, respectively). Also, DE in DFB was greater (P < 0.01) than in PEP2+ (4,935 kcal/kg DM), but ME in DFB was not different from that in PEP2+ (4,617 kcal/kg DM). Furthermore, DE in PEP50 and PEP2+ were greater (P < 0.01) than in fish meal, but ME did not differ from that in fish meal. In Exp. 3, 40 barrows (BW = 12.4 ± 1.3 kg) were randomly allotted to 5 diets with 8 pigs/diet. A P-free diet and 4 diets in which the sole source of P was from DFB, PEP50, PEP2+, or fish meal were formulated. The STTD of P in DFB (96.9%) and PEP2+ (97.6%) were greater (P < 0.01) than in PEP50 and fish meal (76.2% and 68.5%, respectively), and STTD of P in PEP50 was greater (P < 0.01) than in fish meal. In summary, SID of most indispensable AA did not differ among DFB, PEP50, and fish meal, but DE and ME and STTD of P in DFB were greater than in PEP50 and fish meal.
干发酵生物量(DFB)和水解猪肠黏膜分别是 L-赖氨酸盐酸盐生产和肝素提取的副产物。进行了三项实验,以确定 DFB 和两种水解猪肠黏膜产品(PEP50 和 PEP2+)中 AA 的标准回肠可消化率(SID)(实验 1)、DE 和 ME 的浓度(实验 2)以及 P 的标准全肠道消化率(STTD)(实验 3),并将这些值与鱼粉的值进行比较。在实验 1 中,12 头回肠套管的阉公猪(BW=11.5±1.1kg)被分配到 6×6 拉丁方设计的重复中,有 6 种日粮和 6 个周期。设计了一种无氮日粮、基于大豆粉(SBM)的日粮和 4 种基于 SBM 和 DFB、PEP50、PEP2+或鱼粉组合的日粮。除了赖氨酸外,DFB 和鱼粉之间必需氨基酸的SID 没有差异。除了苏氨酸外,PEP50 和鱼粉之间必需氨基酸的 SID 没有差异,但除了赖氨酸和色氨酸外,所有必需氨基酸的 SID 在 PEP2+中都较低(P<0.05)。在实验 2 中,40 头阉公猪(BW=12.8±1.4kg)被分配到 5 种日粮,每 8 头猪/日粮。设计了一种含有 96.4%玉米和 4 种含有玉米和 DFB、PEP50、PEP2+或鱼粉的基础日粮。DFB 的 DE(5,445kcal/kgDM)和 ME(5,236kcal/kgDM)高于 PEP50(DE 和 ME 分别为 4,758 和 4,512kcal/kgDM)和鱼粉(DE 和 ME 分别为 4,227 和 3,960kcal/kgDM)。此外,DFB 的 DE 也高于 PEP2+(P<0.01)(4,935kcal/kgDM),但 ME 与 PEP2+没有差异(4,617kcal/kgDM)。此外,PEP50 和 PEP2+的 DE 高于鱼粉(P<0.01),但 ME 与鱼粉没有差异。在实验 3 中,40 头阉公猪(BW=12.4±1.3kg)被随机分配到 5 种日粮,每 8 头猪/日粮。设计了一种无磷日粮和 4 种以 DFB、PEP50、PEP2+或鱼粉为唯一磷源的日粮。DFB(96.9%)和 PEP2+(97.6%)的 P 标准全肠道消化率高于 PEP50 和鱼粉(分别为 76.2%和 68.5%),PEP50 的 P 标准全肠道消化率也高于鱼粉(P<0.01)。总之,DFB、PEP50 和鱼粉之间大多数必需氨基酸的 SID 没有差异,但 DFB 的 DE 和 ME 以及 P 的 STTD 高于 PEP50 和鱼粉。