MechanoBiology Institute of Singapore, Singapore, Singapore.
PLoS One. 2012;7(8):e44261. doi: 10.1371/journal.pone.0044261. Epub 2012 Aug 31.
Since protein patterning on 2D surfaces has emerged as an important tool in cell biology, the development of easy patterning methods has gained importance in biology labs. In this paper we present a simple, rapid and reliable technique to fabricate thin layers of UV curable polymer with through holes. These membranes are as easy to fabricate as microcontact printing stamps and can be readily used for stencil patterning. We show how this microfabrication scheme allows highly reproducible and highly homogeneous protein patterning with micron sized resolution on surfaces as large as 10 cm(2). Using these stencils, fragile proteins were patterned without loss of function in a fully hydrated state. We further demonstrate how intricate patterns of multiple proteins can be achieved by stacking the stencil membranes. We termed this approach microserigraphy.
由于二维表面上的蛋白质图案已成为细胞生物学中的重要工具，因此在生物学实验室中，开发易于图案化的方法变得越来越重要。在本文中，我们提出了一种简单、快速且可靠的技术，可制造具有通孔的薄的紫外光固化聚合物层。这些膜的制造与微接触印刷印模一样容易，并且可以很容易地用于模版图案化。我们展示了这种微加工方案如何允许在高达 10 cm²的大表面上以微米级分辨率进行高度可重复且高度均匀的蛋白质图案化。使用这些掩模，即使在完全水合状态下，功能也不会丧失，我们仍然可以对易碎蛋白质进行图案化。我们进一步展示了如何通过堆叠掩模膜来实现复杂的多种蛋白质图案。我们将这种方法称为微丝网印刷。
