Mayer I, Borg B, Schulz R
Department of Zoology, University of Stockholm, Sweden.
Gen Comp Endocrinol. 1990 Jan;77(1):70-4. doi: 10.1016/0016-6480(90)90207-3.
Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11 beta-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.
将波罗的海鲑鱼（Salmo salar）、三刺鱼（Gasterosteus aculeatus）、海鲶鱼（Zoarces viviparus）、鲫鱼（Carassius carassius）、非洲鲶鱼（Clarias gariepinus）和非洲长吻鱼（Calamoichthys calabaricus）的血细胞与氚标记的11β-羟基雄烯二酮（OHA）或11-酮基雄烯二酮（OA）一起孵育。在所有鱼类中，OA均转化为11-酮基睾酮（OT），这表明血细胞中存在17β-羟基类固醇脱氢酶活性。另一方面，在任何鱼类中OHA均未转化为11β-羟基睾酮。向孵育物中添加血清在很大程度上阻止了鲑鱼血细胞将OA转化为OT。
