College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430-070, China.
In Vitro Cell Dev Biol Anim. 2012 Sep;48(8):507-17. doi: 10.1007/s11626-012-9543-7. Epub 2012 Sep 6.
The genome size (C value) of eight commercially important fish species in China was measured using flow cytometry. Chicken (Gallus domesticus) erythrocytes were used as reference cells. When using propidium iodide (PI) as the fluorescent dye, genome sizes were 1.09 ± 0.08, 2.75 ± 0.12, 1.05 ± 0.05, 1.35 ± 0.11, 0.99 ± 0.05, 0.90 ± 0.08, 0.90 ± 0.07, and 0.88 ± 0.07 pg for Japanese eel (Anguilla japonica), mullet (Myxocyprinus asiaticus), yellowcheek carp (Elopichthys bambusa), blunt snout bream (Megalobrama amblycephala), yellow catfish (Pelteobagrus fulvidraco), ricefield eel (Monopterus albus), mandarin fish (Siniperca chuatsi), and snakehead (Ophicephalus argus), respectively. However, genome sizes were 1.25 ± 0.00, 3.08 ± 0.02, 1.25 ± 0.00, 1.57 ± 0.01, 0.96 ± 0.01, 1.00 ± 0.01, 0.91 ± 0.01, and 0.89 ± 0.01 pg for these fishes, respectively, when 4', 6-diamidino-2-phenylindole (DAPI) was used as the fluorescent dye. Regardless of the dye used, the more evolutionarily advanced species had a smaller genome size than those with a lower evolutionary status. For each species, we also measured the size of erythrocytes and their nucleus and evaluated the relationships between erythrocyte size, nucleus size, chromosome number, and genome size. Genome size was positively correlated with erythrocyte nucleus size and chromosome number when using PI as the fluorescent dye, but it was only correlated with erythrocyte nucleus size when DAPI was used.
使用流式细胞术测量了中国 8 种商业上重要的鱼类的基因组大小（C 值）。鸡（Gallus domesticus）红细胞被用作参考细胞。当使用碘化丙啶（PI）作为荧光染料时，基因组大小分别为 1.09±0.08、2.75±0.12、1.05±0.05、1.35±0.11、0.99±0.05、0.90±0.08、0.90±0.07 和 0.88±0.07 pg 用于日本鳗（Anguilla japonica）、梭鱼（Myxocyprinus asiaticus）、黄颡鱼（Elopichthys bambusa）、团头鲂（Megalobrama amblycephala）、黄鳝（Pelteobagrus fulvidraco）、稻田鳗（Monopterus albus）、鳜鱼（Siniperca chuatsi）和乌鳢（Ophicephalus argus）。然而，当使用 4'，6-二脒基-2-苯基吲哚（DAPI）作为荧光染料时，这些鱼类的基因组大小分别为 1.25±0.00、3.08±0.02、1.25±0.00、1.57±0.01、0.96±0.01、1.00±0.01、0.91±0.01 和 0.89±0.01 pg。无论使用哪种染料，进化程度较高的物种的基因组大小都小于进化地位较低的物种。对于每种鱼类，我们还测量了红细胞及其核的大小，并评估了红细胞大小、核大小、染色体数与基因组大小之间的关系。使用 PI 作为荧光染料时，基因组大小与红细胞核大小和染色体数呈正相关，但使用 DAPI 时仅与红细胞核大小相关。
