ICAR-Central Marine Fisheries Research Institute, Ernakulam North P.O., Kochi, Kerala, 682018, India.
Nucleome Informatics Pvt. Ltd., NKC Centre for Genomics Research, 2nd Floor, 3 Cube Towers, White Field Rd, HITEC City, Hyderabad, Telangana, 500081, India.
Sci Data. 2024 Aug 28;11(1):930. doi: 10.1038/s41597-024-03802-2.
The Asian green mussel, Perna viridis is an important aquaculture species in the family Mytilidae contributing substantially to molluscan aquaculture. We generated a high-quality chromosome level assembly of this species by combining PacBio single molecule sequencing technique (SMRT), Illumina paired-end sequencing, high-throughput chromosome conformation capture technique (Hi-C) and Bionano mapping. The final assembly resulted in a genome of 723.49 Mb in size with a scaffold N50 of 49.74 Mb with 99% anchored into 15 chromosomes. A total of 49654 protein-coding genes were predicted from the genome. The presence of 634 genes associated with the cancer pathway and 408 genes associated with viral carcinogenesis indicates the potential of this species to be used as a model for cancer studies. The chromosome-level assembly of this species is also a valuable resource for further genomic selection and selective breeding for improving economically important aquaculture traits and augmenting aquaculture productivity.
亚洲青口贻贝(Perna viridis)是贻贝科(Mytilidae)中一种重要的水产养殖物种,对贝类水产养殖业有重要贡献。我们通过结合 PacBio 单分子测序技术(SMRT)、Illumina 配对末端测序、高通量染色体构象捕获技术(Hi-C)和 Bionano 图谱绘制,生成了该物种的高质量染色体水平组装。最终组装得到了一个大小为 723.49Mb 的基因组,支架 N50 为 49.74Mb,99%的序列锚定在 15 条染色体上。从基因组中预测到了 49654 个蛋白质编码基因。该物种存在 634 个与癌症途径相关的基因和 408 个与病毒致癌相关的基因,表明该物种有潜力作为癌症研究的模型。该物种的染色体水平组装也是进一步基因组选择和选择性育种的宝贵资源,可用于改善重要的水产养殖性状和提高水产养殖生产力。