Swathi A, Shekhar M S, Katneni Vinaya Kumar, Vijayan K K
Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R. A. Puram, Chennai, India.
Mol Biol Rep. 2018 Oct;45(5):951-960. doi: 10.1007/s11033-018-4243-3. Epub 2018 Jul 14.
Flow cytometry was used for estimating the genome size of five brackishwater finfish and four shrimp species. The genome size for Lutjanus argentimaculatus was 0.95 ± 0.10 and 0.79 ± 0.01 pg for Scatophagus argus. The genome sizes for Chanos chanos (0.72 ± 0.01 pg), Etroplus suratensis (1.71 ± 0.16 pg) and Liza macrolepis (0.87 ± 0.02 pg) which are important aquaculture species are reported for the first time in this study. The phylogenetic tree constructed using sixty-seven sequence accessions of cytochrome c oxidase subunit 1 (COI) gene of Lates calcarifer revealed two separate clades. The Indian Lates calcarifer species with estimated genome size of 0.44 ± 0.02 pg belonged to a clade different than that of South East Asia and Australia reported to have larger genome size. The genome size for the four major species of genus Penaeus (Penaeus monodon, Penaeus indicus, Penaeus vannamei and Penaeus japonicus) were found in similar range. The genome size of female shrimps ranged from 2.91 ± 0.03 pg (P. monodon) to 2.14 ± 0.02 pg (P. japonicus). In male shrimps, the genome size ranged from 2.86 ± 0.06 pg (P. monodon) to 2.19 ± 0.02 pg (P. indicus). Significant difference was observed in the genome size between male and female shrimp of all species except in P. monodon. The highest relative difference of 12.78% was observed in the genome size between the either sex in P. indicus. The interspecific relative difference of 30.59% in genome size was highest between the male shrimps of P. monodon and P. indicus and 35.98% between the female shrimps of P. monodon and P. japonicus. The stored gills and pleopod tissues could be successfully used up to 3 weeks to estimate the genome size in shrimps.
流式细胞术用于估计五种咸淡水硬骨鱼和四种虾类的基因组大小。紫红笛鲷的基因组大小为0.95±0.10皮克，金钱鱼为0.79±0.01皮克。本研究首次报道了重要水产养殖品种遮目鱼（0.72±0.01皮克）、条纹鲈（1.71±0.16皮克）和大鳞鲻（0.87±0.02皮克）的基因组大小。利用尖吻鲈细胞色素c氧化酶亚基1（COI）基因的67个序列登录构建的系统发育树显示出两个独立的分支。估计基因组大小为0.44±0.02皮克的印度尖吻鲈物种属于一个不同于东南亚和澳大利亚报道的具有更大基因组大小的分支。对虾属的四种主要对虾（斑节对虾、印度对虾、凡纳滨对虾和日本对虾）的基因组大小在相似范围内。雌虾的基因组大小范围从2.91±0.03皮克（斑节对虾）到2.14±0.02皮克（日本对虾）。雄虾的基因组大小范围从2.86±0.06皮克（斑节对虾）到2.19±0.02皮克（印度对虾）。除斑节对虾外，所有物种的雄虾和雌虾在基因组大小上均观察到显著差异。印度对虾两性之间基因组大小的最高相对差异为12.78%。斑节对虾和印度对虾雄虾之间基因组大小的种间相对差异为30.59%，斑节对虾和日本对虾雌虾之间为35.98%。储存的鳃和腹肢组织可成功用于长达3周的时间来估计虾的基因组大小。
