Jafkins Alan, Abu-Daya Anita, Noble Anna, Zimmerman Lyle B, Guille Matthew
European Xenopus Resource Centre, School of Biological Sciences, University of Portsmouth, Portsmouth, England, UK.
Methods Mol Biol. 2012;917:17-31. doi: 10.1007/978-1-61779-992-1_2.
Xenopus tropicalis combine the advantages of X. laevis, for example using explants and targeted gain of function, with the ability to take classical genetics approaches to answering cell and developmental biology questions making it arguably the most versatile of the model organisms. Against this background, husbandry of X. tropicalis is less well developed than for its larger, more robust relative. Here we describe the methods used to keep and breed these frogs successfully.
热带爪蟾兼具非洲爪蟾的优点，例如可使用外植体和定向功能获得法，同时还具备采用经典遗传学方法解答细胞与发育生物学问题的能力，这使得它可以说是最具通用性的模式生物。在此背景下，热带爪蟾的饲养技术相较于其体型更大、更强壮的近亲而言，发展得还不够完善。在此，我们描述了成功饲养和繁殖这些青蛙所使用的方法。
