Center of Comparative Medicine and Pathology, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and Hospital for Special Surgery, New York, New York.
School of Pharmacy, Southwest University, Chongqing, China, and Ural Federal University, Ekaterinburg, Russia.
ILAR J. 2020 Oct 19;60(2):270-288. doi: 10.1093/ilar/ilz019.
The publication of reproducible, replicable, and translatable data in studies utilizing animal models is a scientific, practical, and ethical necessity. This requires careful planning and execution of experiments and accurate reporting of results. Recognition that numerous developmental, environmental, and test-related factors can affect experimental outcomes is essential for a quality study design. Factors commonly considered when designing studies utilizing aquatic animal species include strain, sex, or age of the animal; water quality; temperature; and acoustic and light conditions. However, in the aquatic environment, it is equally important to consider normal species behavior, group dynamics, stocking density, and environmental complexity, including tank design and structural enrichment. Here, we will outline normal species and social behavior of 2 commonly used aquatic species: zebrafish (Danio rerio) and Xenopus (X. laevis and X. tropicalis). We also provide examples as to how these behaviors and the complexity of the tank environment can influence research results and provide general recommendations to assist with improvement of reproducibility and replicability, particularly as it pertains to behavior and environmental complexity, when utilizing these popular aquatic models.
在利用动物模型进行研究时，发表可重复、可复制和可转化的数据是科学、实践和伦理的必要条件。这需要仔细规划和执行实验，并准确报告结果。认识到许多发育、环境和测试相关因素会影响实验结果，对于高质量的研究设计至关重要。在设计利用水生动物物种的研究时，通常会考虑动物的品系、性别或年龄、水质、温度以及声和光条件等因素。然而，在水生环境中，同样重要的是要考虑到正常的物种行为、群体动态、放养密度和环境复杂性，包括水箱设计和结构丰富度。在这里，我们将概述两种常用水生物种（斑马鱼和非洲爪蟾）的正常物种和社会行为。我们还提供了一些例子，说明这些行为和水箱环境的复杂性如何影响研究结果，并提供了一般性建议，以帮助提高这些受欢迎的水生模型的可重复性和可复制性，特别是在行为和环境复杂性方面。
