Rodriguez Isaac A, Madurantakam Parthasarathy A, McCool Jennifer M, Sell Scott A, Yang Hu, Moon Peter C, Bowlin Gary L
Tissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USA.
Int J Biomater. 2012;2012:159484. doi: 10.1155/2012/159484. Epub 2012 Aug 16.
The current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatite : fibrinogen (PDO : nHA : Fg) blended scaffolds in different simulated body fluids (SBF). Scaffolds were fabricated by blending PDO : nHA : Fg in the following percent by weight ratios: 100 : 0 : 0, 50 : 25 : 25, 50 : 50 : 0, 50 : 0 : 50, 0 : 0 : 100, and 0 : 50 : 50. Samples were immersed in (conventional (c), revised (r), ionic (i), and modified (m)) SBF for 5 and 14 days to induce mineralization. Scaffolds were characterized before and after mineralization via scanning electron microscopy, Alizarin Red-based assay, and modified burnout test. The addition of Fg resulted in scaffolds with smaller fiber diameters. Fg containing scaffolds also induced sheet-like mineralization while individual fiber mineralization was noticed in its absence. Mineralized electrospun Fg scaffolds without PDO were not mechanically stable after 5 days in SBF, but had superior mineralization capabilities which produced a thick bone-like mineral (BLM) layer throughout the scaffolds. 50 : 50 : 0 scaffolds incubated in either r-SBF for 5 days or c-SBF for 14 days produced scaffolds with high mineral content and individual-mineralized fibers. These mineralized scaffolds were still porous and will be further optimized as an effective bone substitute in future studies.
目前用于腭裂修复的自体骨移植手术存在一些缺点，如可用性有限、额外的侵入性手术以及供体部位的并发症。本初步研究评估了静电纺丝聚二氧六环酮：纳米羟基磷灰石：纤维蛋白原（PDO：nHA：Fg）混合支架在不同模拟体液（SBF）中的矿化潜力。通过将PDO：nHA：Fg按以下重量百分比比例混合来制备支架：100：0：0、50：25：25、50：50：0、50：0：50、0：0：100和0：50：50。将样品浸入（常规（c）、改良（r）、离子（i）和改性（m））SBF中5天和14天以诱导矿化。通过扫描电子显微镜、基于茜素红的测定和改良的灼烧试验对矿化前后的支架进行表征。添加Fg导致支架的纤维直径更小。含Fg的支架还诱导片状矿化，而在没有Fg的情况下则观察到单个纤维矿化。不含PDO的矿化静电纺丝Fg支架在SBF中放置5天后机械稳定性不佳，但具有优异的矿化能力，在整个支架中产生了一层厚厚的骨样矿物质（BLM）层。在r-SBF中孵育5天或在c-SBF中孵育14天的50：50： 的支架产生了具有高矿物质含量和单个矿化纤维的支架。这些矿化支架仍然多孔，将在未来的研究中作为一种有效的骨替代物进一步优化。
