Ecole Nationale Supérieure Vétérinaire d'Alger, El Harrach, Alger, Algérie.
PLoS One. 2012;7(8):e43621. doi: 10.1371/journal.pone.0043621. Epub 2012 Aug 30.
Whole blood samples from 211 stray cats from Algiers, Algeria, were cultured to detect the presence of Bartonella species and to evaluate the genetic diversity of B. henselae strains by multiple locus VNTR analysis (MLVA). Bartonella henselae was the only species isolated from 36 (17%) of 211 cats. B. henselae genotype I was the predominant genotype (64%). MLVA typing of 259 strains from 30 bacteremic cats revealed 52 different profiles as compared to only 3 profiles using MLST. Of these 52 profiles, 48 (92.3%) were identified for the first time. One-third of the cats harbored one MLVA profile only. As there was a correlation between the age of cats and the number of MLVA profiles, we hypothesized that the single profile in these cats was the profile of the initial infecting strain. Two-third of the cats harbored 2 to 6 MLVA profiles simultaneously. The similarity of MLVA profiles obtained from the same cat, neighbor-joining clustering and structure-neighbor clustering indicate that such a diversity likely results from two different mechanisms occurring either independently or simultaneously: independent infections and genetic drift from a primary strain.
对来自阿尔及利亚阿尔及尔的 211 只流浪猫的全血样本进行培养，以检测巴尔通体物种的存在，并通过多位点可变数量串联重复分析（MLVA）评估亨氏巴尔通体菌株的遗传多样性。从 211 只猫中分离出 36 只（17%）携带巴尔通体。亨氏巴尔通体基因型 I 是主要基因型（64%）。对 30 只菌血症猫的 259 株进行 MLVA 分型，与使用 MLST 相比，仅发现 3 种不同的图谱。在这 52 种图谱中，有 48 种（92.3%）是首次发现。三分之一的猫只携带一种 MLVA 图谱。由于猫的年龄与 MLVA 图谱的数量之间存在相关性，我们假设这些猫的单一图谱是初始感染株的图谱。三分之二的猫同时携带 2 到 6 种 MLVA 图谱。从同一猫身上获得的 MLVA 图谱的相似性、基于邻居joining 的聚类和结构-邻居聚类表明，这种多样性可能是由两种不同的机制独立或同时发生造成的：独立感染和来自原始菌株的遗传漂变。
