Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Plant Signal Behav. 2012 Nov;7(11):1397-401. doi: 10.4161/psb.21902. Epub 2012 Sep 7.
In a recent study we identified corn cystain9 (CC9) as a novel compatibility factor for the interaction of the biotrophic smut fungus Ustilago maydis with its host plant maize. CC9 is transcriptionally induced during the compatible interaction with U. maydis and localizes in the maize apoplast where it inhibits apoplastic papain-like cysteine proteases. The proteases are activated during incompatible interaction and salicylic acid (SA) treatment and, in turn, are sufficient to induce SA signaling including PR-gene expression. Therefore the inhibition of apoplastic papain-like cysteine proteases by CC9 is essential to suppress host immunity during U. maydis infection. Here were present new experimental data on the cysteine protease-cystatin interaction and provide an in silco analysis of plant cystatins and the identified apoplastic cysteine proteases.
在最近的一项研究中，我们发现玉米半胱氨酸蛋白酶抑制剂 9（CC9）是生物亲和性黑粉菌玉米黑粉菌与其宿主植物玉米相互作用的一个新的相容性因子。在与玉米黑粉菌的相容相互作用过程中，CC9 转录诱导，并定位于玉米质外体，在那里它抑制质外体木瓜蛋白酶样半胱氨酸蛋白酶。在不相容相互作用和水杨酸（SA）处理过程中，蛋白酶被激活，反过来，它们足以诱导 SA 信号转导，包括 PR 基因表达。因此，CC9 对质外体木瓜蛋白酶样半胱氨酸蛋白酶的抑制对于抑制玉米黑粉菌感染期间的宿主免疫至关重要。本文提供了关于半胱氨酸蛋白酶-半胱氨酸蛋白酶抑制剂相互作用的新实验数据，并对植物半胱氨酸蛋白酶抑制剂和鉴定的质外体半胱氨酸蛋白酶进行了计算机分析。
