Martinez Manuel, Diaz Isabel
Laboratorio de Bioquímica y Biología Molecular, Dpto, de Biotecnología-Centro de Biotecnología y Genómica de Plantas-Universidad Politécnica de Madrid, ETS Ingenieros Agrónomos, Ciudad Universitaria s/n. 28040 Madrid, Spain.
BMC Evol Biol. 2008 Jul 10;8:198. doi: 10.1186/1471-2148-8-198.
Cystatins and their putative targets, the families of cysteine proteinases C1A and C13 play key roles in plants. Comparative genomic analyses are powerful tools to obtain valuable insights into the conservation and evolution of the proteinases and their proteinaceous inhibitors, and could aid to elucidate issues concerning the function of these proteins.
We have performed an evolutionary comparative analysis of cysteine proteinases C1A and C13 and their putative inhibitors in representative species of different taxonomic groups that appeared during the evolution of the Viridiplantae. The results indicate that whereas C1A cysteine proteinases are present in all taxonomic groups, cystatins and C13 cysteine proteinases are absent in some basal groups. Moreover, gene duplication events have been associated to the increasing structural and functional complexities acquired in land plants.
Comparative genomic analyses have provided us valuable insights into the conservation and evolution of the cystatin inhibitory family and their putative targets, the cysteine proteinases from families C1A and C13. Functionality of both families of proteins in plants must be the result of a coevolutionary process that might have occurred during the evolution of basal and land plants leading to a complex functional relationship among them.
半胱氨酸蛋白酶抑制剂及其假定的靶标，即半胱氨酸蛋白酶C1A和C13家族在植物中发挥着关键作用。比较基因组分析是获取有关蛋白酶及其蛋白质抑制剂的保守性和进化的宝贵见解的有力工具，并且有助于阐明有关这些蛋白质功能的问题。
我们对绿藻门进化过程中出现的不同分类群的代表性物种中的半胱氨酸蛋白酶C1A和C13及其假定的抑制剂进行了进化比较分析。结果表明，虽然C1A半胱氨酸蛋白酶存在于所有分类群中，但某些基部类群中不存在半胱氨酸蛋白酶抑制剂和C13半胱氨酸蛋白酶。此外，基因复制事件与陆地植物中获得的结构和功能复杂性增加有关。
比较基因组分析为我们提供了有关半胱氨酸蛋白酶抑制剂家族及其假定靶标（C1A和C13家族的半胱氨酸蛋白酶）的保守性和进化的宝贵见解。植物中这两个蛋白质家族的功能必定是在基部植物和陆地植物进化过程中可能发生的协同进化过程的结果，这导致了它们之间复杂的功能关系。
