Konecný S, Mícková V
Vet Med (Praha). 1979 Nov;24(11):691-7.
The effect of sulphite-reduction in 33 sample bacterial strains was tested. With regard to the capacity of reducing sulphite in modified sulphite-reduction media in a wide scale of bacterial strains the possibility of an application of selective media with an addition of various concentrations of antibiotic solutions was checked. A concentration of 750 microgram of D-cycloserine per 1 ml of the sulphite-reduction medium appeared to be the most advantageous for the isolation and detection of sulphite-reductive clostridia, above all of Clostridium perfringens. This concentration ensured also a sufficient inhibition of undesirable bacteria without any affection of the growth and capacity of Clostridium perfringens to reduce sulphite in the applied medium.
测试了33株样本细菌菌株的亚硫酸盐还原效果。关于在多种细菌菌株中改良亚硫酸盐还原培养基中还原亚硫酸盐的能力，检查了添加不同浓度抗生素溶液的选择性培养基的应用可能性。每1毫升亚硫酸盐还原培养基中添加750微克D-环丝氨酸的浓度似乎对亚硫酸盐还原梭菌尤其是产气荚膜梭菌的分离和检测最为有利。该浓度还能充分抑制不良细菌，而不会影响产气荚膜梭菌在所用培养基中的生长和还原亚硫酸盐的能力。
