Gubash S M, Ingham L
Provincial Laboratory of Public Health for Southern Alberta, Calgary, Canada.
Zentralbl Bakteriol. 1997 Feb;285(3):397-402. doi: 10.1016/s0934-8840(97)80006-0.
A new differential and selective, bismuth-iron-sulfite-cycloserine (BISC) medium, for isolation and enumeration of Clostridium perfringens from food and feces, was developed. The medium was compared with the widely-used tryptose-sulfite-cycloserine (TSC) medium and blood agar (BA) in recovering actively growing cells, cold- (refrigerated and frozen) stressed, and heat-stressed C. perfringens cells, and heat-activated spores from human feces. Both selective media were satisfactory in recovering actively growing cells and heat-activated spores of C. perfringens. Both were inferior to non-inhibitory blood agar in recovering heat or cold-stressed cells. The advantages of the new BISC medium over the TSC medium were: elimination of the need to prepare pour- or overlay-agar plates, which simplified inoculation of specimens on the medium and simplified the subcultures of colonies for confirmatory identification. All colonies of C. perfringens developed on BISC were black or dark gray. This was contrary to TSC medium, which gave, on average, 39.6% of white colonies when inoculated with the pure cultures of C. perfringens.
开发了一种新型的鉴别性和选择性铋-铁-亚硫酸盐-环丝氨酸（BISC）培养基，用于从食品和粪便中分离和计数产气荚膜梭菌。将该培养基与广泛使用的胰蛋白胨-亚硫酸盐-环丝氨酸（TSC）培养基和血琼脂（BA）进行比较，以回收活跃生长的细胞、冷应激（冷藏和冷冻）及热应激的产气荚膜梭菌细胞，以及从人粪便中回收热激活的孢子。两种选择性培养基在回收活跃生长的产气荚膜梭菌细胞和热激活孢子方面都令人满意。在回收热应激或冷应激细胞方面，两者均不如非抑制性血琼脂。新型BISC培养基相对于TSC培养基的优点是：无需制备倾注或覆盖琼脂平板，这简化了在培养基上接种标本的操作，并简化了用于确证鉴定的菌落传代培养。在BISC上生长的所有产气荚膜梭菌菌落均为黑色或深灰色。这与TSC培养基相反，用产气荚膜梭菌纯培养物接种TSC培养基时，平均有39.6%的白色菌落。
